[en] (15) N NMR relaxation studies, analyses of NMR data to include chemical shifts, residual dipolar couplings (RDC), NOEs and H(N) -H(alpha) coupling constants, and molecular dynamics (MD) simulations have been used to characterise the behaviour of lysozyme from bacteriophage lambda (lambda lysozyme) in solution. The lower and upper lip regions in lambda lysozyme (residues 51-60 and 128-141, respectively) show reduced (1) H-(15) N order parameters indicating mobility on a picosecond timescale. In addition, residues in the lower and upper lips also show exchange contributions to T2 indicative of slower timescale motions. The chemical shift, RDC, coupling constant and NOE data for lambda lysozyme indicate that two fluctuating beta-strands (beta3 and beta4) are populated in the lower lip region while the N terminus of helix alpha6 (residues 136-139) forms dynamic helical turns in the upper lip region. This behaviour is confirmed by MD simulations that show hydrogen bonds, indicative of the beta-sheet and helical secondary structure in the lip regions, with populations of 40-60 %. Thus in solution lambda lysozyme adopts a conformational ensemble that will contain both the open and closed forms observed in the crystal structures of the protein.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Smith, Lorna J.
Bowen, Alice M.
Di Paolo, Alexandre
Matagne, Andre ; Université de Liège - ULiège > Département des sciences de la vie > Enzymologie et repliement des protéines
Redfield, Christina
Language :
English
Title :
The dynamics of lysozyme from bacteriophage lambda in solution probed by NMR and MD simulations.
Publication date :
2013
Journal title :
Chembiochem: A European Journal of Chemical Biology
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