[en] Nowadays synthetic polymers provide more and more functionalities in the biomedical world,
in particular as part of medical devices and drug delivery systems. Looking for new applications in the
cardiovascular field, our attention has been focused on the well-known poly(2-(dimethylamino)ethyl
methacrylate polymer (PDMAEMA). This synthetic polycation has particularly been studied as a
potential DNA carrier to promote cell transfection. However and surprisingly enough relative few
studies have been published in order to better understand its hemoreactivity under a free form (thus not
based on “PolyElectrolyte Complex”), but also its biodistribution and clearance kinetics. In order to
facilitate the in vitro and in vivo monitoring of this polycation and especially to follow its reactivity
with whole blood we have labeled it with fluorescein adopting a new chemical route of synthesis.
After its thorough purification and full-characterization (NMR, SEC, fluorescent spectroscopy),
we followed its interaction with erythrocytes and platelets using flow cytometry. Dose-response
curves were established in whole blood and within incubation times ranging between 5 min to 3 h.
Considering the largest area and more negative Zeta potential developed by red blood cells compared
to platelets, we initially hypothesized that RBC should interact more quickly and efficiently with the
polycation. Unexpectedly, platelets presented a higher affinity for the polycation with a saturation binding curve whilst a linear profile was observed for the erythrocyte adsorption curve.
Aggregometry analyses also revealed this phenomenon, although displaying a weaker effect in whole blood compared to washed platelets.
Ongoing research seeks to understand the molecular mechanism of interaction of this polymer with platelets.
Research Center/Unit :
CEIB - Centre Interfacultaire des Biomatériaux - ULiège
Disciplines :
Hematology
Author, co-author :
Flebus, Luca ; Université de Liège - ULiège > Centre interfacultaire des biomatériaux (CEIB)
Lombart, François ; Université de Liège - ULiège > Centre interfacultaire des biomatériaux (CEIB)
Martinez, Lucia
Defraigne, Jean-Olivier ; Université de Liège - ULiège > Département des sciences cliniques > Chirurgie cardio-vasculaire et thoracique
Delierneux, Céline ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > GIGA-R : Génétique humaine
Oury, Cécile ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > GIGA-R : Génétique humaine
Grandfils, Christian ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, et biochimie humaine
Language :
English
Title :
Study of the specific interaction between fluorescent PDMAEMA and Platelets compared to Erythrocytes