[en] The NLRP3 inflammasome is involved in many obesity-associated diseases such as type 2 diabetes, atherosclerosis and gouty arthritis through its ability to induce IL-1β release. The molecular link between obesity and inflammasome activation is still unclear but free fatty acids have been proposed as one triggering event. Here we reported opposite effects of saturated fatty acids (SFAs) compared to unsaturated fatty acids (UFAs) on NLRP3 inflammasome in human monocytes/macrophages. Palmitate and stearate, both SFAs, triggered IL-1β secretion in a caspase-1/ASC/NLRP3-dependent pathway. Unlike SFAs, the UFAs oleate and linoleate did not lead to IL-1β secretion. In addition, they totally prevented the IL-1β release induced by SFAs and, with less efficiency, by a broad range of NLRP3
inducers including nigericin, alum and MSU. UFAs did not affect the transcriptional effect of SFAs suggesting a specific effect on the NLRP3 activation. These results provide a new antiinflammatory mechanism of UFAs by preventing the activation of the NLRP3 inflammasome and therefore the IL-1β processing. By this way, UFAs might play a protective role in NLRP3-associated diseases.
Unsaturated fatty acids prevent activation of NLRP3 inflammasome in human monocytes/macrophages
Alternative titles :
[fr] Les acides gras insaturés préviennent l'activation de l'inflammasome NLRP3 dans les monocytes/macrophages humains
Publication date :
November 2013
Journal title :
Journal of Lipid Research
ISSN :
0022-2275
eISSN :
1539-7262
Publisher :
Federation of American Societies for Experimental Biology, Bethesda, United States - Maryland
Volume :
54
Pages :
2998-3008
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
This research has been funded by the Interuniversity Attraction Poles (IAP) program initiated by the Belgian Science Policy Office (BELSPO) (IAP grant P7/32)
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