Poster (Scientific congresses and symposiums)
Morphometric analyses of the normal suspensory ligament in Standardbreds
Shikh Al Sook, Mohamad Khir; Antoine, Nadine; Piret, Joëlle et al.
20137th Meeting of the Young Generation of Veterinary Anatomists
 

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Abstract :
[en] The suspensory ligament (SL) is composed of connective tissue (CT) with a variable proportion of muscle (MT) and adipose tissue (AT). The aim of our study is to quantify the CT, MT and AT within the SL in sound horses. Right limbs from 11 horses were collected. Samples from 6 levels of the SL were embedded in paraffin or in Tissue-Tek®. Most of the paraffin sections were shredded. Using the cryosection, some artefacts appeared. Cryoprotection was carried out, which produced the best results. Hematoxylin–phloxine–saffron and Hematoxylin–eosin gave a good contrast of colours between the tissues allowing the use of an image analysis programme. The percentage of MT and AT decreased significantly (P < 0.0001), whereas the percentage of CT increased significantly (P < 0.0001) with age and when descending from the proximal to the distal level of the SL. The percentage of MT was significantly higher (P < 0.0001) in females than males, while the percentage of CT was significantly higher (P < 0.0001) in males than females. The percentage of AT was significantly higher (P = 0.0278) in pelvic limbs than in thoracic limbs. These results confirm the variation in tissue composition within the SL of sound horses.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Shikh Al Sook, Mohamad Khir ;  Université de Liège - ULiège > Doct. sc. vété. (Bologne)
Antoine, Nadine ;  Université de Liège - ULiège > Département de morphologie et pathologie > Histologie
Piret, Joëlle ;  Université de Liège - ULiège > Département de morphologie et pathologie > Département de morphologie et pathologie
Moula, Nassim  ;  Université de Liège - ULiège > Scientifiques attachés au Doyen (F MV)
Busoni, Valeria  ;  Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Imagerie médicale
Denoix, Jean-Marie
Gabriel, Annick ;  Université de Liège - ULiège > Département de morphologie et pathologie > Anatomie des animaux domestiques
Language :
English
Title :
Morphometric analyses of the normal suspensory ligament in Standardbreds
Publication date :
July 2013
Event name :
7th Meeting of the Young Generation of Veterinary Anatomists
Event place :
Leipzig, Germany
Event date :
du 17 juillet 2013 au 20 juillet 2013
Audience :
International
Available on ORBi :
since 07 October 2013

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