Reference : Prevalence and spread of extended-spectrum β-lactamase-producing
Scientific journals : Article
Human health sciences : Public health, health care sciences & services
Human health sciences : Laboratory medicine & medical technology
Human health sciences : Immunology & infectious disease
Prevalence and spread of extended-spectrum β-lactamase-producing
Magoue Lonchel, Carine mailto [Université de Liège - ULiège > > > Doct. sc. médicales (Bologne)]
MELIN, Pierrette mailto [Centre Hospitalier Universitaire de Liège - CHU > > Microbiologie médicale >]
Gangoué-Piéboji, J [Institute of Medical Research and Medicinal Plant Studies, CRPMT, Yaounde, Cameroon > > > >]
Okomo Assoumou, M-C [University of Yaounde, Cameroon > Virology, Faculty of Medicine and Biomedical Sciences > > >]
BOREUX, Raphaël mailto [Centre Hospitalier Universitaire de Liège - CHU > > Microbiologie médicale >]
De Mol, Patrick mailto [Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques >]
Clinical Microbiology and Infection
Blackwell Science
Yes (verified by ORBi)
United Kingdom
[en] Cameroon ; CTX-M-15 ; Enterobacteriaceae ; epidemiology ; extended-spectrum-beta-lactamases
[en] During April 2010 and June 2010, 334 Enterobacteriaceae isolates from 590 participants (outpatients, inpatients, inpatient carers, hospital workers and members of their households) were collected from faecal samples. Based on b-lactamase pattern, origin of strains and the relationship between participants, 44 isolates of extended-spectrum b-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae were selected from 44 participants (in Ngaoundere Protestant Hospital and Ngaoundere Regional Hospital, Cameroon). To determine the relatedness of bacterial strains, these isolates were fingerprinted using the automated, repetitive-sequenced-based PCR-based DiversiLab system. Subsequently, E. coli isolates that had undergone DiversiLab analysis were examined with respect to their phylogenetic group and detection of the ST131 clone to shed light on the epidemiology of these isolates in the Ngaoundere hospitals. The prevalence of faecal carriage of ESBL-producing Enterobacteriaceae among the study participants was 54.06%. According to participant groups, the prevalence of faecal carriage was also high (outpatients 45%; inpatients 67%; inpatient carers 57%; hospital workers 44%; and members of their households 46%). Analysis of the molecular epidemiology of ESBL-producing E. coli and K. pneumoniae showed a close relationship of the isolates between related and nonrelated individuals. In addition, DiversiLab results of E. coli identified four related isolates (4/22) from cluster III belonging to the epidemiologically important clone ST131. Our results highlight the importance of outpatients, inpatients, their carers, hospital workers and their families as reservoirs of ESBLproducing Enterobacteriaceae.
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