Article (Scientific journals)
Prevalence and spread of extended-spectrum β-lactamase-producing
Magoue Lonchel, Carine; MELIN, Pierrette; Gangoué-Piéboji, J et al.
2013In Clinical Microbiology and Infection, 19 (9), p. 416-20
Peer Reviewed verified by ORBi


Full Text
Carine Lonchel 2013 CMI.pdf
Publisher postprint (59.75 kB)

All documents in ORBi are protected by a user license.

Send to


Keywords :
Cameroon; CTX-M-15; Enterobacteriaceae; epidemiology; extended-spectrum-beta-lactamases
Abstract :
[en] During April 2010 and June 2010, 334 Enterobacteriaceae isolates from 590 participants (outpatients, inpatients, inpatient carers, hospital workers and members of their households) were collected from faecal samples. Based on b-lactamase pattern, origin of strains and the relationship between participants, 44 isolates of extended-spectrum b-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae were selected from 44 participants (in Ngaoundere Protestant Hospital and Ngaoundere Regional Hospital, Cameroon). To determine the relatedness of bacterial strains, these isolates were fingerprinted using the automated, repetitive-sequenced-based PCR-based DiversiLab system. Subsequently, E. coli isolates that had undergone DiversiLab analysis were examined with respect to their phylogenetic group and detection of the ST131 clone to shed light on the epidemiology of these isolates in the Ngaoundere hospitals. The prevalence of faecal carriage of ESBL-producing Enterobacteriaceae among the study participants was 54.06%. According to participant groups, the prevalence of faecal carriage was also high (outpatients 45%; inpatients 67%; inpatient carers 57%; hospital workers 44%; and members of their households 46%). Analysis of the molecular epidemiology of ESBL-producing E. coli and K. pneumoniae showed a close relationship of the isolates between related and nonrelated individuals. In addition, DiversiLab results of E. coli identified four related isolates (4/22) from cluster III belonging to the epidemiologically important clone ST131. Our results highlight the importance of outpatients, inpatients, their carers, hospital workers and their families as reservoirs of ESBLproducing Enterobacteriaceae.
Disciplines :
Public health, health care sciences & services
Laboratory medicine & medical technology
Immunology & infectious disease
Author, co-author :
Magoue Lonchel, Carine ;  Université de Liège - ULiège > Doct. sc. médicales (Bologne)
MELIN, Pierrette  ;  Centre Hospitalier Universitaire de Liège - CHU > Microbiologie médicale
Gangoué-Piéboji, J;  Institute of Medical Research and Medicinal Plant Studies, CRPMT, Yaounde, Cameroon
Okomo Assoumou, M-C;  University of Yaounde, Cameroon > Virology, Faculty of Medicine and Biomedical Sciences
BOREUX, Raphaël ;  Centre Hospitalier Universitaire de Liège - CHU > Microbiologie médicale
De Mol, Patrick ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques
Language :
Title :
Prevalence and spread of extended-spectrum β-lactamase-producing
Publication date :
September 2013
Journal title :
Clinical Microbiology and Infection
Publisher :
Blackwell Science, Oxford, United Kingdom
Volume :
Issue :
Pages :
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 26 September 2013


Number of views
104 (11 by ULiège)
Number of downloads
61 (2 by ULiège)

Scopus citations®
Scopus citations®
without self-citations


Similar publications

Contact ORBi