Article (Scientific journals)
CD30-positive peripheral T-cell lymphomas share molecular and phenotypic features
Bisig, B.; de Reyniès, A.; Bonnet, Christophe et al.
2013In Haematologica, 98/n°8, p. 1250-1258
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Keywords :
CD30; Gene expression profiling; Immunophenotyping; Peripheral T-cell lymphoma; T-cell receptor
Abstract :
[en] Peripheral T-cell lymphoma, not otherwise specified is a heterogeneous group of aggressive neoplasms with indistinct borders. By gene expression profiling we previously reported unsupervised clusters of peripheral T-cell lymphomas, not otherwise specified correlating with CD30 expression. In this work we extended the analysis of peripheral T-cell lymphoma molecular profiles to prototypical CD30+ peripheral T-cell lymphomas (anaplastic large cell lymphomas), and validated mRNA expression profiles at the protein level. Existing transcriptomic datasets from peripheral T-cell lymphomas, not otherwise specified and anaplastic large cell lymphomas were reanalyzed. Twenty-one markers were selected for immunohistochemical validation on 80 peripheral T-cell lymphoma samples (not otherwise specified, CD30+ and CD30–; anaplastic large cell lymphomas, ALK+ and ALK–), and differences between subgroups were assessed. Clinical follow-up was recorded. Compared to CD30– tumors, CD30+ peripheral T-cell lymphomas, not otherwise specified were significantly enriched in ALK– anaplastic large cell lymphoma-related genes. By immunohistochemistry, CD30+ peripheral T-cell lymphomas, not otherwise specified differed significantly from CD30– samples [down-regulated expression of T-cell receptor-associated proximal tyrosine kinases (Lck, Fyn, Itk) and of proteins involved in T-cell differentiation/activation (CD69, ICOS, CD52, NFATc2); upregulation of JunB and MUM1], while overlapping with anaplastic large cell lymphomas. CD30– peripheral T-cell lymphomas, not otherwise specified tended to have an inferior clinical outcome compared to the CD30+ subgroups. In conclusion, we show molecular and phenotypic features common to CD30+ peripheral T-cell lymphomas, and significant differences between CD30– and CD30+ peripheral T-cell lymphomas, not otherwise specified, suggesting that CD30 expression might delineate two biologically distinct subgroups.
Disciplines :
Hematology
Author, co-author :
Bisig, B.
de Reyniès, A.
Bonnet, Christophe ;  Université de Liège - ULiège > GIGA-R : Hématologie
Sujobert, P.
Rickman, D.S.
Marafioti, T.
Delsol, G.
Lamant, L.
Gaulard, P.
de Leval, L.
Language :
English
Title :
CD30-positive peripheral T-cell lymphomas share molecular and phenotypic features
Publication date :
2013
Journal title :
Haematologica
ISSN :
0390-6078
eISSN :
1592-8721
Publisher :
Ferrata Storti Foundation, Pavia, Italy
Volume :
98/n°8
Pages :
1250-1258
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 08 September 2013

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