[en] Objectives: To determine the point prevalence of carbapenem-non-susceptible Enterobacteriaceae (CNSE) and carbapenemase-producing Enterobacteriaceae (CPE) isolates among hospitalized patients in Belgium.
Methods: Twenty-four hospital-based laboratories prospectively collected 200 non-duplicated Enterobacteriaceae isolates from clinical specimens of hospitalized patients over a 2 month period. All isolates were screened locally for decreased susceptibility to carbapenem drugs using a disc diffusion method according to CLSI interpretative criteria. CNSE strains were referred centrally for confirmation of carbapenemase by phenotypic and molecular testing.
Results: From February to April 2012, 158 of the 4564 screened Enterobacteriaceae isolates were categorized as non-susceptible to carbapenems, resulting in a point prevalence of CNSE of 3.5% (95% CI: 2.9%–4.2%; range per centre: 0.5%–8.5%). Of the 125 referred CNSE isolates, 11 Klebsiella pneumoniae isolates [OXA-48 (n=7), KPC type (n=3) and NDM type (n=1)], 1 OXA-48-positive Escherichia coli isolate and 1 KPC-positive Klebsiella oxytoca isolate were detected in eight hospitals. None of the 72 carbapenem-non-susceptible Enterobacter spp. isolates were confirmed as CPE. The minimal estimated point prevalence of CPE isolates was 0.28% (13/ 4564; 95% CI: 0.13%–0.44%) overall (range per centre: 0%–1.5%).
Conclusions: Despite the overall low prevalence of CNSE found in this study, the detection of CPE isolates in one-third of the participating centres raises concerns and highly suggests the spread and establishment of CPE in Belgian hospitals.
Research Center/Unit :
National Reference Laboratory for Monitoring of Antimicrobial Resistance in Gram-negative Bacteria
Disciplines :
Laboratory medicine & medical technology Public health, health care sciences & services Immunology & infectious disease
Author, co-author :
Huang, Te-Din; Université Catholique de Louvain - UCL > CHU Mont-Godinne > National Reference Laboratory for Monitoring of Antimicrobial Resistance in Gram-negative Bacteria
Berhin, Catherine; Université Catholique de Louvain - UCL > CHU Mont-Godinne > National Reference Laboratory for Monitoring of Antimicrobial Resistance in Gram-negative Bacteria
Glupczynski, Youri; Université Catholique de Louvain - UCL > CHU Mont-Godinne > National Reference Laboratory for Monitoring of Antimicrobial Resistance in Gram-negative Bacteria
MELIN, Pierrette ; Centre Hospitalier Universitaire de Liège - CHU > Microbiologie médicale
Multicentre Study Group
Language :
English
Title :
Prevalence and mechanisms of resistance to carbapenems in Enterobacteriaceae
Publication date :
August 2013
Journal title :
Journal of Antimicrobial Chemotherapy
ISSN :
0305-7453
eISSN :
1460-2091
Publisher :
Oxford University Press, London, United Kingdom
Volume :
68
Issue :
8
Pages :
1832-7
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
INAMI - Institut National d'Assurance Maladie-Invalidité
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