Article (Scientific journals)
Glycoprotein B cleavage is important for murid herpesvirus 4 to infect myeloid cells.
Glauser, Daniel L.; Milho, Ricardo; Frederico, Bruno et al.
2013In Journal of Virology
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Abstract :
[en] Glycoprotein B (gB) is a conserved herpesvirus virion component implicated in membrane fusion. As with many - but not all - herpesviruses, the gB of murid herpesvirus 4 (MuHV-4) is cleaved into disulfide-linked subunits, apparently by furin. Preventing gB cleavage for some herpesviruses causes minor infection deficits in vitro, but what the cleavage contributes to host colonization has been unclear. To address this we mutated the furin cleavage site (R-R-K-R) of the MuHV-4 gB. Abolishing gB cleavage did not affect its expression levels, glycosylation or antigenic conformation. In vitro, mutant viruses entered fibroblasts and epithelial cells normally, but had a significant entry deficit in myeloid cells such as macrophages and bone marrow-derived dendritic cells. The deficit in myeloid cells was not due to reduced virion binding or endocytosis, suggesting that gB cleavage promotes infection at a post-endocytic entry step, presumably viral membrane fusion. In vivo, viruses lacking gB cleavage showed reduced lytic spread in the lungs. Alveolar epithelial cell infection was normal, but alveolar macrophage infection was significantly reduced. Normal long-term latency in lymphoid tissue was established nonetheless.
Disciplines :
Microbiology
Author, co-author :
Glauser, Daniel L.
Milho, Ricardo
Frederico, Bruno
May, Janet S.
Kratz, Anne-Sophie
Gillet, Laurent  ;  Université de Liège - ULiège
Stevenson, Philip G.
Language :
English
Title :
Glycoprotein B cleavage is important for murid herpesvirus 4 to infect myeloid cells.
Publication date :
2013
Journal title :
Journal of Virology
ISSN :
0022-538X
eISSN :
1098-5514
Publisher :
American Society for Microbiology (ASM), Washington, United States - District of Columbia
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 21 August 2013

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