Article (Scientific journals)
New linear lipopeptides produced by pseudomonas cichorii SF1-54 are involved in virulence, swarming motility, and biofilm formation
Pauwelyn, E.; Huang, C. J.; Ongena, Marc et al.
2013In Molecular Plant-Microbe Interactions, 26 (5), p. 585-598
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Abstract :
[en] Pseudomonas cichorii is the causal agent of lettuce midrib rot, characterized by a dark-brown to green-black discoloration of the midrib. Formation of necrotic lesions by several plant-pathogenic pseudomonads is associated with production of phytotoxic lipopeptides, which contribute to virulence. Therefore, the ability of P. cichorii SF1-54 to produce lipopeptides was investigated. A cell-free culture filtrate of SF1-54 showed surfactant, antimicrobial, and phytotoxic activities which are typical for lipopeptides. High-performance liquid chromatography analysis of P. cichorii SF1-54 culture filtrate revealed the presence of seven compounds with lipopeptide characteristics. Two related lipopeptides, named cichofactin A and B, were studied in more detail: they are linear lipopeptides with a decanoic and dodecanoic lipid chain, respectively, connected to the N-terminus of an eight-amino-acid peptide moiety. Both cichofactins are new members of the syringafactin lipopeptide family. Furthermore, two nonribosomal peptide synthethase-encoding genes, cifA and cifB, were identified as responsible for cichofactin biosynthesis. A cifAB deletion mutant no longer produced cichofactins and was impaired in swarming motility but showed enhanced biofilm formation. Upon spray inoculation on lettuce, the cichofactindeficient mutant caused significantly less rotten midribs than the wild type, indicating that cichofactins are involved in pathogenicity of P. cichorii SF1-54. Further analysis revealed that P. cichorii isolates vary greatly in swarming motility and cichofactin production. © 2013 The American Phytopathological Society.
Disciplines :
Phytobiology (plant sciences, forestry, mycology...)
Author, co-author :
Pauwelyn, E.
Huang, C. J.
Ongena, Marc ;  Université de Liège - ULiège
Leclère, V.
Jacques, Philippe ;  Université de Liège - ULiège > Chimie et bio-industries > Bio-industries
Bleyaert, P.
Budzikiewicz, H.
Schäfer, M.
Höfte, M.
Language :
English
Title :
New linear lipopeptides produced by pseudomonas cichorii SF1-54 are involved in virulence, swarming motility, and biofilm formation
Publication date :
2013
Journal title :
Molecular Plant-Microbe Interactions
ISSN :
0894-0282
eISSN :
1943-7706
Publisher :
American Phytopathological Society, St Paul, United States - Minnesota
Volume :
26
Issue :
5
Pages :
585-598
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 08 July 2013

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