[en] We developed dual paclitaxel (PTX)/superparamagnetic iron oxide (SPIO)-loaded PLGA-based nanoparticles for a theranostic purpose. Nanoparticles presented a spherical morphology and a size of 240 nm. The PTX and iron loading were 1.84 ± 0.4 and 10.4 ± 1.93 mg/100 mg respectively. Relaxometry studies and phantom MRI demonstrated their efficacy as T2 contrast agent. Significant cellular uptake by CT26 cells of nanoparticles was shown by Prussian blue staining and fluorescent microscopy. While SPIO did not show any toxicity in CT-26 cells, PTX-loaded nanoparticles had a cytotoxic activity. PTX-loaded nanoparticle (5 mg/kg) with or without co-encapulated SPIO induced in vivo a regrowth delay of CT26 tumors. Together these multifunctional nanoparticles may be considered as future nanomedicine for simultaneous molecular imaging, drug delivery and real-time monitoring of therapeutic response.
Research Center/Unit :
Center for Education and Research on Macromolecules (CERM)
Disciplines :
Chemistry Materials science & engineering
Author, co-author :
Schleich, N.; Université catholique de Louvain (UCL), Brussels > Louvain Drug Research Institute (LDRI) > Pharmaceutics and Drug Delivery
Sibret, Pierre ; Université de Liège - ULiège > Department of Chemistry > Center for Education and Research on Macromolecules (CERM)
Danhier, P.; Université catholique de Louvain (UCL) > Louvain Drug Research Institute (LDRI) > Laboratory of Biomedical Magnetic Resonance
Ucakar, B.; Université catholique de Louvain (UCL), Brussels > Louvain Drug Research Institute (LDRI) > Pharmaceutics and Drug Delivery
Laurent, S.; University of Mons (UMons) > Department of General, Organic, and Biomedical Chemistry > NMR and Molecular Imaging Laboratory
Muller, R.; University of Mons (UMons) > Department of General, Organic, and Biomedical Chemistry > NMR and Molecular Imaging Laboratory > CMMI, Center for Microscopy and Molecular Imaging, Gosselies, Belgium
Jérôme, Christine ; Université de Liège - ULiège > Department of Chemistry > Center for Education and Research on Macromolecules (CERM)
Gallez, Bernard; Université catholique de Louvain (UCL) > Louvain Drug Research Institute (LDRI) > Laboratory of Biomedical Magnetic Resonance
Préat, Véronique; Université catholique de Louvain (UCL), Brussels > Louvain Drug Research Institute (LDRI) > Pharmaceutics and Drug Delivery
Danhier, F.; Université catholique de Louvain (UCL), Brussels > Louvain Drug Research Institute (LDRI) > Pharmaceutics and Drug Delivery
Language :
English
Title :
Dual anticancer drug/superparamagnetic iron oxide-loaded PLGA-based nanoparticles for cancer therapy and magnetic resonance imaging
T. Allkemper, C. Bremer, L. Matuszewski, W. Ebert, and P. Reimer Contrast-enhanced blood-pool MR angiography with optimized iron oxides: effect of size and dose on vascular contrast enhancement in rabbits Radiology 223 2002 432 438
A. Bjornerud, and L. Johansson The utility of superparamagnetic contrast agents in MRI: theoretical consideration and applications in the cardiovascular system NMR Biomed. 17 2004 465 477
K. Brindle New approaches for imaging tumour responses to treatment Nat. Rev. Cancer 8 2008 94 107
F. Danhier, N. Lecouturier, B. Vroman, C. Jérôme, J. Marchand-Brynaert, O. Feron, and V. Préat Paclitaxel-loaded PEGylated PLGA-based nanoparticles: in vitro and in vivo evaluation J. Control. Release 133 2009 11 17
F. Danhier, B. Vroman, N. Lecouturier, N. Crokart, V. Pourcelle, H. Freichels, C. Jérôme, J. Marchand-Brynaert, O. Feron, and V. Préat Targeting of tumor endothelium by RGD-grafted PLGA-nanoparticles loaded with paclitaxel J. Control. Release 140 2009 166 173
F. Danhier, O. Feron, and V. Préat To exploit the tumor microenvironment: passive and active tumor targeting of nanocarriers for anti-cancer drug delivery J. Control. Release 148 2010 135 146
F. Danhier, E. Ansorena, J.M. Silva, R. Coco, A. Le Breton, and V. Préat PLGA-based nanoparticles: an overview of biomedical applications J. Control. Release 161 2012 505 522
P. Danhier, G. De Preter, S. Boutry, I. Mahieu, P. Leveque, J. Magat, V. Haufroid, P. Sonveaux, C. Bouzin, O. Feron, R.N. Muller, B.F. Jordan, and B. Gallez Electron paramagnetic resonance as a sensitive tool to assess the iron oxide content in cells for MRI cell labeling studies Contrast Media Mol. Imaging 7 2012 302 307
V. Fievez, L. Plapied, R.A. des, V. Pourcelle, H. Freichels, V. Wascotte, M.L. Vanderhaeghen, C. Jérôme, A. Vanderplasschen, J. Marchand-Brynaert, Y.J. Schneider, and V. Préat Targeting nanoparticles to M cells with non-peptidic ligands for oral vaccination Eur. J. Pharm. Biopharm. 73 2009 16 24
H. Freichels, F. Danhier, V. Préat, P. Lecomte, and C. Jérôme Fluorescent labeling of degradable poly(lactide-co- glycolide) for cellular nanoparticles tracking in living cells Int. J. Artif. Organs 34 2011 152 160
M. Garinot, V. Fievez, V. Pourcelle, F. Stoffelbach, A. des Rieux, L. Plapied, I. Theate, H. Freichels, C. Jérôme, J. Marchand-Brynaert, Y.J. Schneider, and V. Préat PEGylated PLGA-based nanoparticles targeting M cells for oral vaccination J. Control. Release 120 2007 195 204
J.S. Guthi, S.G. Yang, G. Huang, S. Li, C. Khemtong, C.W. Kessinger, M. Peyton, J.D. Minna, K.C. Brown, and J. Gao MRI-visible micellar nanomedicine for targeted drug delivery to lung cancer cells Mol. Pharmacol. 7 2010 32 40
M. Hamoudeh, F.A. Al, E. Canet-Soulas, F. Bessueille, D. Leonard, and H. Fessi Elaboration of PLLA-based superparamagnetic nanoparticles: characterization magnetic behaviour study and in vitro relaxivity evaluation Int. J. Pharm. 338 2007 248 257
J. Hu, Y. Qian, X. Wang, T. Liu, and S. Liu Drug-loaded and superparamagnetic iron oxide nanoparticle surface-embedded amphiphilic block copolymer micelles for integrated chemotherapeutic drug delivery and MR imaging Langmuir 28 2012 2073 2082
T. Lammers, F. Kiessling, W.E. Hennink, and G. Storm Nanotheranostics and image-guided drug delivery: current concepts and future directions Mol. Pharmacol. 7 2010 1899 1912
S. Laurent, J.L. Bridot, L.V. Elst, and R.N. Muller Magnetic iron oxide nanoparticles for biomedical applications Future. Med Chem. 2 2010 427 449
P.W. Lee, S.H. Hsu, J.J. Wang, J.S. Tsai, K.J. Lin, S.P. Wey, F.R. Chen, C.H. Lai, T.C. Yen, and H.W. Sung The characteristics biodistribution, magnetic resonance imaging and biodegradability of superparamagnetic core-shell nanoparticles Biomaterials 31 2010 1316 1324
Y. Ling, K. Wei, Y. Luo, X. Gao, and S. Zhong Dual docetaxel/ superparamagnetic iron oxide loaded nanoparticles for both targeting magnetic resonance imaging and cancer therapy Biomaterials 32 2011 7139 7150
Y. Ling, K. Wei, F. Zou, and S. Zhong Temozolomide loaded PLGA-based superparamagnetic nanoparticles for magnetic resonance imaging and treatment of malignant glioma Int. J. Pharm. 430 2012 266 275
J. Lu, S. Ma, J. Sun, C. Xia, C. Liu, Z. Wang, X. Zhao, F. Gao, Q. Gong, B. Song, X. Shuai, H. Ai, and Z. Gu Manganese ferrite nanoparticle micellar nanocomposites as MRI contrast agent for liver imaging Biomaterials 30 2009 2919 2928
H. Maeda, J. Wu, T. Sawa, Y. Matsumura, and K. Hori Tumor vascular permeability and the EPR effect in macromolecular therapeutics: a review J. Control. Release 65 2000 271 284
R. Massart Preparation of aqueous magnetic liquids in alkaline and acidic media IEEE Transactions on Magnetics 17 1981 1247 1248
T. Mosmann Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays J. Immunol. Methods 65 1983 55 63
R.A. Petros, and J.M. Desimone Strategies in the design of nanoparticles for therapeutic applications Nat. Rev. Drug Discov. 9 2010 615 627
V. Pourcelle, S. Devouge, M. Garinot, V. Preat, and J. Marchand-Brynaert PCL-PEG-based nanoparticles grafted with GRGDS peptide: preparation and surface analysis by XPS Biomacromolecules 8 2007 3977 3983
E. Raymond, A. Hanauske, S. Faivre, E. Izbicka, G. Clark, E.K. Rowinsky, and D.D. Von Hoff Effects of prolonged versus short-term exposure paclitaxel (Taxol) on human tumor colony-forming units Anticancer Drugs 8 1997 379 385
A. Roch, R.N. Muller, and P. Gillis Theory of proton relaxation induced by superparamagnetic particles J. Chem. Phys. 110 1999 5403 5411
A. Roch, Y. Gossuin, R.N. Muller, and P. Gillis Superparamagnetic colloid suspensions: water magnetic relaxation and clustering J. Magn. Magn. Mater. 293 2005 532 539
P.B. Schiff, J. Fant, and S.B. Horwitz Promotion of microtubule assembly in vitro by taxol Nature 277 1979 665 667
M.J. Shieh, C.Y. Hsu, L.Y. Huang, H.Y. Chen, F.H. Huang, and P.S. Lai Reversal of doxorubicin-resistance by multifunctional nanoparticles in MCF-7/ADR cells J. Control. Release 152 2011 418 425
A. Singh, F. Dilnawaz, S. Mewar, U. Sharma, N.R. Jagannathan, and S.K. Sahoo Composite polymeric magnetic nanoparticles for co-delivery of hydrophobic and hydrophilic anticancer drugs and MRI imaging for cancer therapy ACS Appl. Mater. Interfaces 3 2011 842 856
A.K. Singla, A. Garg, and D. Aggarwal Paclitaxel and its formulations Int. J. Pharm. 235 2002 179 192
M. Talelli, C.J. Rijcken, T. Lammers, P.R. Seevinck, G. Storm, C.F. van Nostrum, and W.E. Hennink Superparamagnetic iron oxide nanoparticles encapsulated in biodegradable thermosensitive polymeric micelles: toward a targeted nanomedicine suitable for image-guided drug delivery Langmuir 25 2009 2060 2067
R.B. Weiss, R.C. Donehower, P.H. Wiernik, T. Ohnuma, R.J. Gralla, D.L. Trump, J.R. Baker Jr.; D.A. Van Echo, D.D. Von Hoff, and B. Leyland-Jones Hypersensitivity reactions from taxol J. Clin. Oncol. 8 1990 1263 1268
X. Yang, J.J. Grailer, I.J. Rowland, A. Javadi, S.A. Hurley, D.A. Steeber, and S. Gong Multifunctional SPIO/DOX-loaded wormlike polymer vesicles for cancer therapy and MR imaging Biomaterials 31 2010 9065 9073
G.A. van Ewijk, G.J. Vroege, and A.P. Philipse Convenient preparation methods for magnetic colloids J. Magn. Magn. Mater. 201 1999 31 33
