Article (Scientific journals)
Hypoxia is not required for human endometrial breakdown or repair in a xenograft model of menstruation.
Coudyzer, Pauline; Lemoine, Pascale; Jordan, Benedicte F. et al.
2013In FASEB Journal, sous presse
Peer Reviewed verified by ORBi
 

Files


Full Text
CoudyzerP Faseb 2013.pdf
Publisher postprint (555.79 kB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
estradiol and progesterone; hypoxia-induced factor 1-α; matrix metalloproteinases; mouse model; pimonidazole
Abstract :
[en] Menstrual endometrial breakdown induced by estradiol and progesterone withdrawal is regularly attributed to vasospasm of spiral arteries causing ischemia and hypoxia. We investigated whether hypoxia actually occurred in an in vivo model of menstruation. Three complementary approaches were used to look for signs of hypoxia in fragments of human functionalis xenografted to ovariectomized immunodeficient mice bearing pellets-releasing estradiol and progesterone, and then deprived of ovarian steroids. Hormone withdrawal 21 d after grafting induced menstrual breakdown and MMP expression within 4 d. Local partial oxygen pressure (pO2) was measured by electron paramagnetic resonance using implanted lithium phtalocyanine crystals. In mice with hormone maintenance until sacrifice, pO2 was low one week after grafting (14.8+/-3.4 mmHg) but increased twofold from the second week when tissue was largely revascularized. After 3 wk, pO2 was not modified by hormone withdrawal but was slightly increased on hormone reimpregnation 4 d after removal (34.7+/-6.1 mmHg) by comparison with hormone maintenance (27.1+/-8.6 mmHg). These results were confirmed using fluorescence quenching-based OxyLite measurements. In a further search for signs of hypoxia, we did not find significant HIF1-alpha immunostaining, nor pimonidazole adducts after hormone withdrawal. We conclude that hypoxia is not needed to trigger menstrual-like tissue breakdown or repair in human endometrial xenograft.-Coudyzer, P., Lemoine, P., Jordan, B. F., Gallez, B., Galant, C., Nisolle, M., Courtoy, P. J., Henriet, P., and Marbaix, E. Hypoxia is not required for human endometrial breakdown or repair in a xenograft model of menstruation.
Disciplines :
Reproductive medicine (gynecology, andrology, obstetrics)
Author, co-author :
Coudyzer, Pauline
Lemoine, Pascale
Jordan, Benedicte F.
Gallez, Bernard
Galant, Christine
Nisolle, Michelle ;  Université de Liège - ULiège > Département des sciences cliniques > Gynécologie - Obstétrique
Courtoy, Pierre J.
Henriet, Patrick
Marbaix, Etienne
Language :
English
Title :
Hypoxia is not required for human endometrial breakdown or repair in a xenograft model of menstruation.
Publication date :
2013
Journal title :
FASEB Journal
ISSN :
0892-6638
eISSN :
1530-6860
Publisher :
Federation of American Societies for Experimental Biology, United States - Maryland
Volume :
sous presse
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 17 June 2013

Statistics


Number of views
88 (4 by ULiège)
Number of downloads
4 (4 by ULiège)

Scopus citations®
 
19
Scopus citations®
without self-citations
18
OpenCitations
 
15

Bibliography


Similar publications



Contact ORBi