Article (Scientific journals)
Acinetobacter is more prevalent than Asaia in natural populations of the mosquito vector Aedes albopictus with isolates showing diverse genomic architecture and substrate utilization.
Minard, Guillaume; Tran, Florence Hélène; Raharimalala, Fara Nantenaina et al.
2013In FEMS Microbiology Ecology, 83, p. 63–73
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Minard, 2012 Prevalence, genomic and metaboloi profiles of Acinetobacter and Asaia in Aedes albopictus from Madagascar.pdf
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Keywords :
acetic bacteria; asian tiger mosquito; symbiosis; genomic diversity; metabolic repertoire
Abstract :
[en] The presence of cultivable bacteria Acinetobacter and Asaia was recently demonstrated in the mosquito vector Aedes albopictus. However, it is not known how prevalent these bacteria are in field populations. Here, the presence of these bacteria in Ae. albopictus populations from Madagascar was diagnosed by amplification of 16S rRNA gene fragments. Both genera were detected at relatively high frequencies, 46% for Asaia and 74% for Acinetobacter. The prevalence of Acinetobacter correlated significantly with mosquito gender, and the prevalence of Asaia with the interaction between mosquito gender and the sampling site. For each bacterial genus, more male than female mosquitoes were infected. Using pulse field gel electrophoresis, no significant difference in genome size was found between Acinetobacter isolates from mosquitoes compared with free-living Acinetobacter. However, a great diversity was observed in plasmid numbers (from 1 to 12) and sizes (from < 8 to 690 kb). Mosquito isolates utilized fewer substrates than free-living isolates, but some substrates known as blood or plant components were specifically utilized by mosquito isolates. Therefore it is likely that a specific subpopulation of Acinetobacter is selected by Ae. albopictus. Overall, this study emphasizes the need to gain a global view on the bacterial partners in mosquito vectors
Research center :
Université Claude Bernard Lyon 1
Université d'Antananarivo
Disciplines :
Entomology & pest control
Microbiology
Author, co-author :
Minard, Guillaume;  Université Claude Bernard - Lyon 1 - UCLB > Ecologie Microbienne UMR 5557 > 2013
Tran, Florence Hélène;  Université Claude Bernard - Lyon 1 - UCLB > Ecologie Microbienne UMR 5557 > 2013
Raharimalala, Fara Nantenaina ;  Université d'Antananarivo - Université Claude Bernard Lyon 1 > Sciences Naturelles > Entomologie - Ecologie Microbienne UMR 5557 > 2013
Hellard, Eléonore;  Université Claude Bernard - Lyon 1 - UCLB > 2013
Ravelonandro, Pierre;  Centre Nationale de Recherche et de l'Environnement (CNRE) > Entomologie > 2013
Mavingui, Patrick;  Université Claude Bernard - Lyon 1 - UCLB > Ecologie Microbienne UMR 5557 > 2013
Valiente Moro, Claire;  Université Claude Bernard - Lyon 1 - UCLB > Ecologie Microbienne UMR 5557 > 2013
Language :
English
Title :
Acinetobacter is more prevalent than Asaia in natural populations of the mosquito vector Aedes albopictus with isolates showing diverse genomic architecture and substrate utilization.
Publication date :
2013
Journal title :
FEMS Microbiology Ecology
ISSN :
0168-6496
eISSN :
1574-6941
Publisher :
Blackwell Publishing, Oxford, United Kingdom
Volume :
83
Pages :
63–73
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
Arthropod Symbioses: from fundamental to pest disease management
Funders :
FRB-CD-AOOI-07-10
GDRI ‘Biodiversité et Développement Durable à Madagascar’
COST action F0701 ‘Arthropod Symbioses: from fundamental to pest disease management’
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