[en] Multiplex PCR was used to detect Fasciola gigantica infection in Lymnaea viridis snail as intermediate host. In this study, snail Lymnaea viridis was experimentally infected with F.gigantica and used for DNA template. Fasciola specific primers was amplified a 124 bp fragment in multiplex PCR when the genomic DNA isolated from F.gigantica infected Lymnaea viridis snails was used as template and amplified a 500 - 600 bp fragment. Genomic DNA of the parasite was used as a positive control, which also gave an amplification of the 124 bp fragment. DNA isolated from non-infected snails was used as a negative control and no amplification of this sequence was observed. The developed diagnostic multiplex PCR will be of use for assessment of transmission of fascioliasis in snail as intermediate host in Vietnam.
