Poster (Scientific congresses and symposiums)
Pili genes pattern in Group B streptococci from newborn infections and pregnant women in Europe (DEVANI Project)
Imperi, Monica; Rinaudo, Daniela; Creti, Roberta et al.
2011Lancefield 2011, XVIIIth LISSSD
 

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Keywords :
Group B streptococcus; pili
Abstract :
[en] Objectives Evaluation of the presence and expression of genes coding for pili in a collection of group B streptococcci (GBS) isolated from newborn infection and pregnant women in the course of the DEVANI (Design of a Vaccine Against Neonatal Infection) project. Methods GBS isolates from pregnant women (PW) and cases of newborn infection (NI) were collected in 8 European countries (Belgium, Bulgaria, Czech Republic, Denmark, Germany, Italy, Spain, United Kingdom) during 2009/10 under the auspices of DEVANI. Total no. of strains examined was 1078 and 192 from PW and NI, respectively. Isolates were screened by multiplex PCR and FACS analysis to evaluate respectively gene presence and surface-exposure of pili. Results The most common gene patterns found were PI-2a alone, PI 1+2a and PI 1+2b, while the PI-2b gene alone was very rare. The most prominent result was that a majority of isolates from NI carried the PI-1+2b gene pattern, while the most common pattern among PW was PI-1+2a. Most of analyzed strains express at least one pilus on their surface. Conclusions All isolates contained at least one gene coding for pili. When present pili 2a and 2b were highly surface exposed.
Disciplines :
Immunology & infectious disease
Laboratory medicine & medical technology
Author, co-author :
Imperi, Monica;  Istituto Superiore di Sanità, Rome - Italy
Rinaudo, Daniela;  Novartis Vaccines & Diagnostics, Siena - Italy
Creti, Roberta;  Istituto Superiore di Sanità, Rome - Italy
Pataracchia, Marco;  Istituto Superiore di Sanità, Rome - Italy
Rosini, Roberto;  Novartis Vaccines & Diagnostics, Siena - Italy
Nuccitelli, Annalisa;  Novartis Vaccines & Diagnostics, Siena - Italy
Kriz, Paula;  National Institute of Public Health, Prague, Czech Republic
Kilian, Mogens;  Aarhus University, Denmark > Department of Medical Microbiology and Immunology
Hufnagel, Markus;  University Medical Center Freiburg - Germany
Efstratiou, Androulla;  Health Protection Agency, Colindale - UK
de la Rosa, Manuel;  Hospital Universitario Virgen de las Nieves, Granada - Spain
MELIN, Pierrette  ;  Centre Hospitalier Universitaire de Liège - CHU > Microbiologie médicale
Detcheva, Antoaneta;  National Center of Infectious and Parasitic Diseases, Sofia, Bulgaria
Baldassarri, Lucilla;  Istituto Superiore di Sanità, Rome - Italy
Maione, Domenico;  Novartis Vaccines & Diagnostics, Siena - Italy
More authors (5 more) Less
Language :
English
Title :
Pili genes pattern in Group B streptococci from newborn infections and pregnant women in Europe (DEVANI Project)
Publication date :
September 2011
Event name :
Lancefield 2011, XVIIIth LISSSD
Event place :
Palerme, Italy
Event date :
du 4 septembre 2011 au 8 septembre 2011
Audience :
International
European Projects :
FP7 - 200481 - DEVANI - Design of a vaccine to immunize neonates against GBS infections through a durable maternal immune response
Funders :
CE - Commission Européenne [BE]
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since 04 May 2013

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