Reference : Human papillomavirus capsids trigger crosstalk between dendritic and NK cells
Scientific congresses and symposiums : Poster
Human health sciences : Immunology & infectious disease
http://hdl.handle.net/2268/147651
Human papillomavirus capsids trigger crosstalk between dendritic and NK cells
English
Langers, Inge mailto [Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
Renoux, Virginie []
Pirotte, Evelyne []
Dortu, Estelle [Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
BONIVER, Jacques [Centre Hospitalier Universitaire de Liège - CHU > > Anatomie pathologique >]
DELVENNE, Philippe [Centre Hospitalier Universitaire de Liège - CHU > > Anatomie pathologique >]
Jacobs, Nathalie mailto [Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
26-Sep-2012
No
International
Natural Killer Cell Symposium 2012
from 26-09-2012 to 28-09-2012
Heidelberg
Germany
[en] human papillomavirus ; natural killer cells ; dendritic cells
[en] The immune system controls, at least partially, human papillomavirus (HPV) infection and subsequent tumour development as demonstrated by a higher tumour prevalence in immunodeficient patients. More than 90% of HPV-infected women will clear the virus within two years. However, it remains unclear which immune cells are implicated in this process and although dendritic cells (DC) and NK cells play a key role in host resistance to virus and tumour, no study has been performed evaluating their crosstalk in this context.
Virus-like particles (VLP) formed by the HPV major capsid protein L1 are licensed as vaccine against cervical cancer and we have recently shown that NK cells can directly interact with these HPV-VLP [1]. Here, we investigated the impact of this activation on NK-DC crosstalk. Interestingly, NK cells increase DC maturation induced by HPV-VLP as shown by an up-regulation of HLA-DR and CD86 on DC. Transwell experiments indicated that the expression of HLA-DR is cell-cell contact and soluble factor dependent, whereas only soluble factors seem to be required for CD86 expression. Moreover, in the presence of HPV-VLP and NK cells, DC produce higher amounts of IL12p70, while the production of the immunosuppressive cytokine IL10 remains unchanged. We also demonstrated that DC can up-regulate the expression of NK activation markers (CD69 and HLA-DR) in the presence of HPV-VLP. This up-regulation requires both cell-cell contact and soluble factors. Regarding HLA-DR marker, the increased expression on CD56bright cells is mediated by soluble factors, whereas cell-cell contacts are also important for HLA-DR expression on CD56dim cells. In the presence of DC activated by HPV-VLP, the function of NK cells is also modified since they become more cytotoxic against HPV+ cell line and secrete more IFN-γ.
Our results suggest that NK-DC crosstalk could play a role in the immune response induced by HPV-VLP during vaccination protocols against cervical cancer.
Giga-Infection, Immunity and Inflammation
Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS ; Televie
Researchers ; Students
http://hdl.handle.net/2268/147651

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