In vitro ototoxicity of aminoglycosides and platin derivatives.  A semi-automatic assay for sensory hair cell damage in explanted rat organ of corti.

Malgrange, B.; LEFEBVRE, Philippe; van de Water, T.R.; Bonnet, Christophe; Monville, F.; Rigo, J.-M.; Staecker, H.; Moonen, Gustave

doi:10.1016/S0887-2333(98)00048-4

Article (Scientific journals)

In vitro ototoxicity of aminoglycosides and platin derivatives. A semi-automatic assay for sensory hair cell damage in explanted rat organ of corti.

Malgrange, B.; LEFEBVRE, Philippe; van de Water, T.R. et al.

1998 • In Toxicology in Vitro, 12 (6), p. 705-714

Peer Reviewed verified by ORBi

Permalink
https://hdl.handle.net/2268/146348

DOI
10.1016/S0887-2333(98)00048-4

Files (1)Send to Details Statistics Bibliography Similar publications

Files

Full Text

Toxicol In Vitro-In vitro ototoxicity of... 1998.pdf

Publisher postprint (1.01 MB)

Download

All documents in ORBi are protected by a user license.

Send to

RIS BibTex APA Chicago Permalink X Linkedin

Details

Keywords :

Cochlea; Aminoglycosides; Cisplatin; Ototoxicity; Otoprotection

Abstract :

[en] The ototoxic damage that drugs such as neomycin, kanamycin, colistin, cisplatin, transplatin and carboplatin cause on outer and inner hair cells in postnatal day 3 rat cochlear explants was investigated. Phalloidin-fluorescein conjugate-stained stereocilia bundles of sensory hair cells were quantified by video image analysis as a measurement of ototoxic effect. The video image quantification system established dose-response curves for ototoxic drugs (e.g. calculation of an IC50) and allowed comparisons between several ototoxins from the same family. This methodology provided the means to assess the efficacy of otoprotectant agents in preventing ototoxicity. Poly-l-aspartate (10-5M) and poly-l-glutamate (10-5M) protected auditory hair cells from neomycin (10-3M) toxicity while reduced glutathione (10-3M) provided protection against cisplatin (10-4M)-induced hair cell damage.

Disciplines :

Hematology

Author, co-author :

Malgrange, B.

LEFEBVRE, Philippe ; Centre Hospitalier Universitaire de Liège - CHU > O.R.L.

van de Water, T.R.

Bonnet, Christophe ; Université de Liège - ULiège > GIGA-R : Hématologie

Monville, F.

Rigo, J.-M.

Staecker, H.

Moonen, Gustave ; Université de Liège - ULiège > Département des sciences cliniques > Département des sciences cliniques

Language :

English

Title :

In vitro ototoxicity of aminoglycosides and platin derivatives. A semi-automatic assay for sensory hair cell damage in explanted rat organ of corti.

Publication date :

1998

Journal title :

Toxicology in Vitro

ISSN :

0887-2333

eISSN :

1879-3177

Publisher :

Pergamon-Elsevier Science Ltd, Oxford, United Kingdom

Volume :

12 (6)

Pages :

705-714

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 08 April 2013

Statistics

Number of views

90 (1 by ULiège)

Number of downloads

308 (1 by ULiège)

More statistics

Scopus citations^®

Scopus citations^®
without self-citations

OpenCitations

OpenAlex citations

Bibliography

Bottenstein J. E. and Sato G. (1979) Growth of rat neuroblastoma cell line in serum-free supplemented medium. Proceedings of the National Academy of Sciences of the U.S.A. 76, 514-517.
Cascinu S., Cordella L., Del Ferro E., Fronzoni M. and Catalano G. (1995) Neuroprotective effect of reduced glutathione on cisplatin-based chemotherapy in advanced gastric cancer: a randomized double-blind placebo-controlled trial. Journal of Clinical Oncology 13, 26-32.
Cavaletti G., Cascinu S., Venturino P., Tedeschi M. and Tredici G. (1996) Neuroprotectant drugs in cisplatin neurotoxicity. Anticancer Research 16, 3149-3160.
Cavaletti G., Minoia C., Schieppati M. and Tredici G. (1994) Protective effects of glutathione on cisplatin neurotoxicity in rats. International Journal of Radiation, Oncology, Biology and Physics 29, 771-776.
Cavaletti G., Tredici G., Pizzini G., Marmiroli P., Fabbrica D. and Mincia R. (1992) Morphologic, morphometric and toxicologic evaluations of the effect of cisplatin alone or in combination with glutathione on the rat nervous system. Clinical Neuropharmacology 11, 169-169.
Chiodo A. A. and Alberti P. W. (1994) Experimental, clinical and preventive aspects of ototoxicity. (Review). European Archives of Oto-Rhino-Laryngology 251, 375-392.
Colombo N., Bini S., Miceli D., Bogliun G., Marzorati G., Cavaletti G., Parmigiani F., Venturino P., Tedeschi M., Frattola L., Buratti C. and Mangioni C. (1995) Weekly cisplatin +/- glutathione in relapsed ovarian carcinoma. International Journal of Gynecological Cancer 5, 81-86.
Di Re F., Bohm S., Oriana S., Spatti G. B., Pirovano C., Tedeschi M. and Zunino F. (1993) High-dose cisplatin and cyclophosphamide with glutathione in the treatment of advanced ovarian cancer. Annals of Oncology 4, 55-61.
Gandara D. R., Perez E. A., Weibe V. and De Gregorio M. W. (1991) Cisplatin chemoprotection and rescue: pharmacologic modulation of toxicity. (Review). Seminars in Oncology 18, 49-55.
Hamers F. P. T., Brakkee J. H., Cavalletti E., Tedeschi M., Marmonti L., Pezzoni G., Neijt J. P. and Gispen W. H. (1993) Reduced glutathione protects against cisplatin-induced neurotoxicity in rats. Cancer Research 53, 544-549.
Hangfu M., Zhao J. and Din D. (1992) The prophylactic effect of thyroxin on kanamycin ototoxicity in guinea pigs. Hearing Research 61, 132-136.
Harpur E. S. (1981) Ototoxicity. In In Vitro Methods in Toxicology. Edited by C. K. Atterwill and C. E. Steele, pp. 37-58. Cambridge University Press, London.
Harpur E. S. (1987) The inner ear. In Target Organ Toxicity. Edited by G. M. Cohen, pp. 125-142. CRC Press, Boca Raton, FL.
Harpur E. S. (1989) Ototoxicity: morphological and functional correlations between experimental and clinical studies. In Perspectives in Basic and Applied Toxicology. Edited by B. Ballantyne, pp. 42-69. Wright, London.
Hulka G. F., Prazma J., Brownlee R. E., Pulver S. and Pillsbury H. C. (1993) Use of poly-1-aspartic acid to inhibit aminoglycoside cochlear ototoxicity. American Journal of Otology 14, 352-356.
Koegel L. (1985) Ototoxicity: a contemporary review of aminoglycosides, loop duretics, acetylsalicylic acid, quinine, erythromycin, and cisplatinum. American Journal of Otology 6, 190-199.
Kotecha B. and Richardson G. P. (1994) Ototoxicity in vitro: effects of neomycin, gentamicin, dihydrostreptomycin, amikacin, spectinomycin, neamine, spermine and poly-L-lysine. Hearing Research 73, 173-184.
Kroese A. B. A. and Van den Bercken J. (1980) Dual action of ototoxic antibiotics on sensory hair cells. Nature 283, 395-397.
Lefebvre P. P., Demanez S., Weber T. and Moonen G. (1989) Trophic and toxic effects on cultured hair cells and spiral ganglia neurons. Acta Otolaryngologica Belgica 43, 39-40.
Lefebvre P. P., Malgrange B., Staecker H., Moonen G. and Van De Water T. R. (1993) Retinoic acid stimulates regeneration of mammalian auditory hair cells after ototoxic damage in vitro. Science 260/5108, 692-694.
Lefebvre P. P., Weber T., Rigo J-M., Delrée P., Leprince P. and Moonen G. (1990) Potassium-induced release of an endogenous toxic activity for outer hair cell and auditory neurons in the cochlea: a new pathophysiological mechanism in Ménière's disease? Hearing Research 47, 83-94.
Morin J. P., Viotte G., Vandewalle A., Van Hoof F., Tulkens P. and Fillastre J. P. (1980) Gentamicin-induced nephrotoxicity: a cell biology approach. Kidney International 18, 583-590.
Pierson M. G. and Moller A. R. (1981) Prophylaxis of kanamycin-induced ototoxicity by a radioprotectant. Hearing Research 4, 79-87.
Ramsammy L., Josepovitz C., Lane B. and Kaloyanides J. (1990) Polyaspartic acid inhibits gentamicin-induced perturbations of phospholipid metabolism. American Journal of Physiology 259, C1141-C1149.
Ramsammy L., Josepovitz C., Lane B. P. and Kaloyanides G. J. (1988) Polyaspartic acid protects against gentamicin nephrotoxicity in the rat. Journal of Pharmacology and Experimental Therapeutics 1, 149-153.
Richardson G. P. and Russell I. J. (1991) Cochlear cultures as a model for studying aminoglycoside induced ototoxicity. Hearing Research 53, 293-311.
Ryals B. M. and Rubel E. W. (1988) Hair cell regeneration after acoustic trauma in adult coturnix quail. Science 240, 1774-1776.
Rybak L. P., Ravi R. and Somani S. M. (1995) Mechanism of protection by diethyldithiocarbamate against cisplatin ototoxicity: antioxidant system. Fundamental and Applied Toxicology 26, 293-300.
Saito T., Saito H., Saito K., Wakui S., Manabe Y. and Tsuda G. (1989) Ototoxicity of carboplatin in guinea pigs. Auris Nasus Larynx 16, 13-21.
Schacht J. (1986) Molecular mechanisms of drug-induced hearing loss. Hearing Research 22, 297-304.
Schweitzer V. G., Rarey K. E., Dolan D. F. and Abrams G. (1986) Ototoxicity of cisplatin vs. platinum analogs CBDCA (JM-8) and CHIP (JM-9). Otolaryngology, Head and Neck Surgery 94, 458-470.
Takada A. and Schacht J. (1982) Calcium antagonism and reversibility of gentamicin-induced loss of coch́lear microphonics in guinea pig. Hearing Research 8, 179-186.
Taudy M., Syka J., Popelár J. and Ulehlová L. (1992) Carboplatin and cisplatin ototoxicity in guinea pigs. Audiology 31, 293-299.
Tran Ba Huy P., Manuel C., Meulemans A., Sterkers O. and Amiel C. (1981) Pharmacokinetics of gentamicin in perilymph and endolymph of the rat as determined by radioimmunoassay. Journal of Infectious Diseases 143, 476-486.
Tredici G., Cavaletti G., Petruccioli M. G., Fabbrica D., Tedeschi M. and Venturino P. (1994) Low-dose glutathione administration in the prevention of cisplatin-induced peripheral neuropathy in rats. Neurotoxicology 15, 701-704.
Tulkens P. M. (1989) Nephrotoxicity of aminoglycoside antibiotics. Toxicology Letters 46, 107-123.
Wersäll J. (1995) Ototoxic antibiotics: a review. Acta Otolaryngology (Stockholm) 519, 26-29.
Williams P. D. and Hottendorf G. H. (1985) Inhibition of renal membrane binding and nephrotoxicity of gentamicin by polyasparagine and polyaspartic acid in the rat. Research Communications in Chemical Pathology and Pharmacology 47, 317-320.
Williams P. D., Hottendorf G. H. and Bennett D. B. (1986) Inhibition of renal membrane binding and nephrotoxicity of aminoglycosides. Journal of Pharmacology and Experimental Therapeutics 237, 919-925.
Williams S. E., Zenner H. P. and Schacht J. (1987) Three molecular steps of aminoglycoside ototoxicity demonstrated in outer hair cells. Hearing Research 30, 11-18.