Impact of bone marrow-derived mesenchymal stromal cells on experimental xenogeneic graft-versus-host disease

[en] Background aims. Graft-versus-host disease (GVHD) is a life-threatening complication of allogeneic hematopoietic cell transplantation caused by donor T cells reacting against host tissues. Previous studies have suggested that mesenchymal stromal cells (MSCs) could exert potent immunosuppressive effects. Methods. The ability of human bone marrow derived MSCs to prevent xenogeneic GVHD in non-obese diabetic/severe combined immunodeficient (NOD/SCID) mice and in NOD/SCID/interleukin-2Rg(null) (NSG) mice transplanted with human peripheral blood mononuclear cells (PBMCs) was assessed. Results. Injection of 200 106 human PBMCs intraperitoneally (IP) into sub-lethally (3.0 Gy) irradiated NOD/SCID mice also given anti-asialo GM1 antibodies IP 1 day prior and 8 days after transplantation induced lethal xenogeneic GVHD in all tested mice. Co-injection of 2 106 MSCs IP on day 0 did not prevent lethal xenogeneic GVHD induced by injection of human PBMCs. Similarly, injection of 30 106 human PBMCs IP into sub-lethally (2.5 Gy) irradiated NSG mice induced a lethal xenogeneic GVHD in all tested mice. Injection of 3 106 MSCs IP on days 0, 7, 14 and 21 did not prevent lethal xenogeneic GVHD induced by injection of human PBMCs. Conclusions. Injection of MSCs did not prevent xenogeneic GVHD in these two humanized mice models.

Disciplines :

Hematology

Author, co-author :

Bruck, France ^✱

Belle, Ludovic ^✱; Université de Liège > GIGA-R : Hématologie

LECHANTEUR, Chantal ; Centre Hospitalier Universitaire de Liège - CHU > Thérapie cellulaire

de Leval, Laurence

Hannon, Muriel ; Université de Liège - ULiège > GIGA-R : Hématologie

DUBOIS, Sophie ; Centre Hospitalier Universitaire de Liège - CHU > Thérapie cellulaire

CASTERMANS, Emilie ; Centre Hospitalier Universitaire de Liège - CHU > Génétique

Humblet-Baron, Stéphanie

Rahmouni, Souad ; Université de Liège - ULiège > Département des sciences cliniques > GIGA-R:Immunopath. - Maladies infect. et médec. inter. gén.

Beguin, Yves ; Université de Liège - ULiège > GIGA-R : Hématologie

BRIQUET, Alexandra ^✱; Centre Hospitalier Universitaire de Liège - CHU > Centre d'oncologie

Baron, Frédéric ^✱; Université de Liège - ULiège > GIGA-R : Hématologie

^✱ These authors have contributed equally to this work.

Language :

English

Title :

Impact of bone marrow-derived mesenchymal stromal cells on experimental xenogeneic graft-versus-host disease

Publication date :

March 2013

Journal title :

Cytotherapy

ISSN :

1465-3249

eISSN :

1477-2566

Publisher :

Taylor & Francis, London, United Kingdom

Volume :

Issue :

Pages :

267-279

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 13 March 2013

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