Action potential of the median giant fibre of the earthworm, lumbricus terrestris, as a pharmacological test of the radio-labelled TTX-derivatives activity
Daemers-Lambert, Claire; Grandfils, Christian
1988 • In Comparative Biochemistry and Physiology. C, Comparative Pharmacology, 90C (1), p. 107-112
[en] The isolated median giant fibre action potential from the earthworm nerve cord was recorded in a double sucrose-gap apparatus used in current-clamp configuration. [3H]Lysine-TTX was synthesized and its inhibitory effect on the spike area was measured. Its activity is very similar to that of TTX. The dose-response curve obtained with superthreshold stimuli is shown to be the right-hand side of the Kd/(Kd+[TTX]) relation. A 3.9 nM Kd is calculated which agrees with the known values for the frog Ranvier node.
Research center :
Laboratoire d'Océanologie et Laboratoire de Biochimie Générale et Comparée
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Daemers-Lambert, Claire; Université de Liège - ULiège > Océanologie
Grandfils, Christian ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, et biochimie humaine
Language :
English
Title :
Action potential of the median giant fibre of the earthworm, lumbricus terrestris, as a pharmacological test of the radio-labelled TTX-derivatives activity
Publication date :
1988
Journal title :
Comparative Biochemistry and Physiology. C, Comparative Pharmacology
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