atomic force microscopy; DNA; gene therapy vehicle; Dynamic Light Scattering
Abstract :
[en] Atomic force microscopy (AFM) is used to describe the formation process of polymer/DNA complexes. Two main objectives of this research are presented. The first one is to apply AFM as an effective tool to analyse DNA molecules and different polycation/DNA complexes in order to evaluate their degree of condensation (size and shape). The other one is to search for a relationship between the condensation state of DNA and its transfection efficiency. In this study, linear methacrylate based polymers and globular SuperFect polymers are used in order to induce DNA condensation. Ternary complexes, composed of methacrylate based polymers and polyethylene glycol (PEG)-based copolymers, are also investigated. AFM allows us to confirm good condensation conditions and relate them (or not) to transfection efficiencies. These AFM results (obtained after drying in air) are compared with measurements deduced from Dynamic Light Scattering (DLS) experiments performed in water. This comparison allowed us to identify the structural modifications resulting from deposition on the mica surface. (c) 2006 Elsevier B.V. All rights reserved.
Research Center/Unit :
CEIB - Centre Interfacultaire des Biomatériaux - ULiège
Disciplines :
Microbiology Biotechnology
Author, co-author :
Volcke, C.
Pirotton, S.
Grandfils, Christian ; Université de Liège - ULiège > Biochimie et physiologie générale
Humbert, C.
Thiry, P. A.
Ydens, I.
Dubois, Philippe ; Université de Liège - ULiège > Centre d'études et de rech. sur les macromolécules (CERM)
Raes, M.
Language :
English
Title :
Influence of DNA condensation state on transfection efficiency in DNA/polymer complexes: An AFM and DLS comparative study
Publication date :
20 August 2006
Journal title :
Journal of Biotechnology
ISSN :
0168-1656
eISSN :
1873-4863
Publisher :
Elsevier Science Bv, Amsterdam, Netherlands
Volume :
125
Issue :
1
Pages :
11-21
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
Nouveaux polycations et vectorisation d’ADN dans les cellules de parois vasculaires” Convention Région Wallonne n° 14612, année 2001-2004 dans le cadre du concours Initiative 2000
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