Reference : Optimization and Application of Electrophoretic Mobility Analysis of Human Red Blood ...
Scientific journals : Article
Human health sciences : Hematology
http://hdl.handle.net/2268/143482
Optimization and Application of Electrophoretic Mobility Analysis of Human Red Blood Cells to Study Their in Vitro Stability, Interaction with Polycations and Proteolytic Enzymes
English
Grandfils, Christian mailto [Université de Liège - ULiège > > Biochimie et physiologie générale >]
Foresto, P. [> > > >]
Riquelme, B. [> > > >]
Valverde, J. [> > > >]
Sondag-Thull, D. [> > > >]
Feb-2008
Journal of Biomedical Materials Research. Part A
84
2
535-44
Yes (verified by ORBi)
International
1549-3296
[en] Zeta potential ; Red blood cell ; membrane
[en] Electrophoretic light scattering method has been considered to determine both the mean and polydispersity of electrophoretic mobility of normal human red blood cells. The final goal of our study was to optimize an in vitro test allowing us to investigate the interaction of xenobiotics, in particular polyelectrolytes, with blood cells. The feasibility of our method has been evaluated based on the reproducibility of our technique to analyze the native electrophoretic mobility of human RBCs, as well as their evolution in the presence of polycationic compounds, i.e. a natural polyamine, spermine, and a synthetic polycation, a polyethylenimine (PEI). Additionally, the follow-up of the human RBCs electrophoretic mobility has been controlled after their incubation with neuraminidase.
Centre Interfacultaire des Biomatériaux - CEIB
Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS
http://hdl.handle.net/2268/143482
10.1002/jbm.a.31354

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