Modelling Lymphatic and blood capillary patterning

modeling; lymphatic and blood capillary; culture models, of vessel formation; angio- and lymphangiogenesis; n vitro models, of angio and lymph-angiogenesis; culturing isolated endothelial cells; in vitro dedifferentiation; LEC monoculture suitability; ring assays; aorta and lymphatic ring assays; 2D- and 3D-culture systems

Abstract :

[en] Angiogenesis and lymphangiogenesis, the formation of new blood or lymphatic vessels from preexisting ones, are important biological processes associated with diverse physiological processes, tissue repair and pathologies, such as cancer. Much progress has been made in recent years in identifying molecules specifically expressed on blood and lymphatic vessels and in the setting up of models of angiogenesis and lymphangiogenesis. In this review, we describe the most common in vitro models of (lymph)angiogenesis that have proven suitable for investigating angiogenic and lymphatic biology, and offer alternatives to animal experimentation. Their rationales, limitations and applications in biomedical research are discussed. A special emphasis will be given on ring assays that provide excellent recapitulation of various steps of (lymph)angiogenesis. The aortic ring assay has become the most widely used methods to study in vitro angiogenesis, and the recently set up lymphatic ring assay provides the opportunity to extend the in vitro studies to lymphangiogenesis.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Bruyere, Françoise

Maillard, Catherine ; Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement

Erpicum, Charlotte ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie cellulaire et moléculaire appliquée à l'homme

Noël, Agnès ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie cellulaire et moléculaire appliquée à l'homme

Language :

English

Title :

Modelling Lymphatic and blood capillary patterning

Publication date :

2012

Main work title :

Replacing Animal Models, a practical guide to creating and using culture-based biomimetic alternatives

Editor :

Davies, Jamie

Publisher :

Wiley-Blackwell

ISBN/EAN :

9780470974254

Pages :

45-56

Peer reviewed :

Peer reviewed

Available on ORBi :

since 21 February 2013

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66 (15 by ULiège)

Number of downloads

4 (4 by ULiège)

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