Article (Scientific journals)
Capsular Gene Typing of Streptococcus agalactiae Compared to Serotyping by Latex Agglutination
Yao, Kaihu; Poulsen, Knud; Maione, Domenico et al.
2012In Journal of Clinical Microbiology, 51 (2), p. 503-507
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Keywords :
Streptococcus agalactiae; Capsular gene typing; serotyping
Abstract :
[en] We evaluated three different PCR-based capsular gene typing methods applied to 312 human and bovine Streptococcus agalac- tiae (group B Streptococcus [GBS]) isolates and compared the results to serotyping results obtained by latex agglutination. Among 281 human isolates 27% could not be typed by latex agglutination. All 312 isolates except 5 could be typed by the three PCR methods combined. Two of these methods were multiplex assays. Among the isolates that were typeable by both latex ag- glutination and capsular gene typing, 94% showed agreement between the two methods. However, each of the PCR methods showed limitations. One of the methods did not include all 10 recognized serotypes, one misidentified eight isolates of serotypes Ib and IV as serotype Ia, and one did not distinguish between serotypes VII and IX. For five isolates that showed aberrant pat- terns in the capsular gene typing, long-range PCR targeting the cps operon disclosed large insertions or deletions affecting the cps gene cluster. A sensitive flow cytometric assay based on serotype-specific antibodies applied to 76 selected isolates that were nontypeable by latex agglutination revealed that approximately one-half of these did express capsular polysaccharide. A proce- dure for convenient and reliable capsular gene typing to be included in epidemiological and surveillance studies of S. agalactiae is proposed.
Research center :
Department of Biomedicine, Faculty of Health Sciences, Aarhus University, Aarhus, Denmark
Disciplines :
Laboratory medicine & medical technology
Immunology & infectious disease
Author, co-author :
Yao, Kaihu;  Aarhus University, Aarhus, Denmark > Department of Biomedicine, Faculty of Health Sciences
Poulsen, Knud;  Aarhus University, Aarhus, Denmark > Department of Biomedicine, Faculty of Health Sciences
Maione, Domenico;  Novartis Vaccines and Diagnostics, Siena, Italy > R&D
Rinaudo, Daniela;  Novartis Vaccines and Diagnostics, Siena, Italy > R&D
Baldassarri, Lucilla;  Instituto Superiore di Sanita, Rome, Italy > Dipartimento di Malattie Infettive, Parassitarie ed Immunomediate
Telford, John;  Novartis Vaccines and Diagnostics, Siena, Italy
Skov Sørensen, Uffe B.;  Aarhus University, Aarhus, Denmark > Department of Biomedicine, Faculty of Health Sciences
Members of the DEVANI Study Group
Kilian, Mogens;  Aarhus University, Aarhus, Denmarka > Department of Biomedicine, Faculty of Health Sciences
Other collaborator :
Melin, Pierrette  ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Bactériologie, mycologie, parasitologie, virologie
Language :
English
Title :
Capsular Gene Typing of Streptococcus agalactiae Compared to Serotyping by Latex Agglutination
Publication date :
28 November 2012
Journal title :
Journal of Clinical Microbiology
ISSN :
0095-1137
eISSN :
1098-660X
Publisher :
American Society for Microbiology (ASM), Washington, United States - District of Columbia
Volume :
51
Issue :
2
Pages :
503-507
Peer reviewed :
Peer Reviewed verified by ORBi
European Projects :
FP7 - 200481 - DEVANI - Design of a vaccine to immunize neonates against GBS infections through a durable maternal immune response
Funders :
CE - Commission Européenne [BE]
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