Reference : HTLV-1 clonality during chronic infection and BLV clonality during primary infection
Scientific congresses and symposiums : Paper published in a journal
Life sciences : Genetics & genetic processes
http://hdl.handle.net/2268/141534
HTLV-1 clonality during chronic infection and BLV clonality during primary infection
English
Gillet, N. []
Hlela, C. []
Verdonck, T. []
Gotuzzo, E. []
Clark, D. []
Rodriguez, Sabrina [Université de Liège - ULiège > Chimie et bio-industries > Biologie cell. et moléc. >]
Malani, N. []
Melamed, A. []
Gormley, N. []
Carter, R. []
Bentley, D. []
Berry, C. []
Bushman, F.D. []
Taylor, G.P. []
Willems, L. []
Bangham, C.R.M. []
6-Jun-2011
AIDS Research and Human Retroviruses
Mary Ann Liebert, Inc.
8
1
A185
Yes (verified by ORBi)
International
0889-2229
1931-8405
Larchmont
NY
15th International Conference on Human Retrovirology: HTLV and Related Viruses
from 5-06-2011 to 8-06-2011
International Retrovirology Association
Leuven and Gembloux
Belgium
[en] HTLV-1 clonality during chronic infection and BLV clonality during primary infection
Nicolas A Gillet1,2*, Carol Hlela1, Tine Verdonck3, Eduardo Gotuzzo3, Daniel Clark3, Sabrina Rodriguez2, Nirav Malani4, Anat Melamed1, Niall Gormley5, Richard Carter5, David Bentley5, Charles Berry6, Frederic D Bushman4, Graham P Taylor7, Luc Willems2, Charles R M Bangham1
1Department of Immunology, Wright-Fleming Institute, Imperial College London, London, W2 1PG, UK.
2Molecular and Cellular Epigenetics, Interdisciplinary Cluster for Applied Genoproteomics (GIGA) of University of Liège (ULg), Liège, 4000, Belgium.
3Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Peru.
4Department of Microbiology, University of Pennsylvania School of Medicine, Pennsylvania, Philadelphia, PA, 19104, USA.
5Illumina, Chesterford Research Park, Essex, Little Chesterford, CB10 1XL, UK.
6University of California, California, La Jolla San Diego, CA, 92093-0901, USA.
7Department of Genitourinary Medicine and Communicable Diseases, Wright-Fleming
Institute, Imperial College London, London, W2 1PG, UK.


HTLV-1 persists by driving clonal proliferation of infected T-lymphocytes. A high proviral load predisposes to the inflammatory and malignant diseases associated with HTLV-1. Yet the reasons for the remarkable variation within and between individuals in the abundance of HTLV-1-infected clones remain unknown. We demonstrate that negative selection dominates during chronic infection, favouring establishment of proviruses integrated in transcriptionally silenced DNA: this selection is significantly stronger in asymptomatic carriers.
We postulated that this selection occurred mainly during the primary infection. We are testing this hypothesis in an animal model by studying the BLV clonality during the primary infection in cows. By measuring the proviral load, the anti-BLV immune response and the BLV clonality we aim to quantify the impact of the immune response on the rate of infectious spread and on the selection of proviruses inserted in a particular genomic environment. Co-infection with Strongyloides stercoralis or Staphylococcus appears to be another risk factor for the development of HTLV-1 associated diseases.
We observed that HTLV-1 clonality is altered by co-infection with these pathogens with an increase of both the number and the abundance of the infected T-cell clones. The genomic characteristics of the proviral integration sites in the most abundant clones differ significantly between co-infected individuals and those with HTLV-1 alone, implying the existence of different selection forces in co-infected patients. The rate of appearance of new clones in patients co-infected with Strongyloides stercoralis is higher than in patients with HTLV-1 alone. By comparing skin lesions and blood samples from patients with Infective Dermatitis associated with HTLV-1 (IDH), we observed a significant proportion of distinct infected clones between the two compartments. The skin lesions seem to be a site for HTLV-1 infectious spread.
Researchers
http://hdl.handle.net/2268/141534

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Restricted access
Clonality HTLV.pdfAuthor preprint9.64 kBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.