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Detection of BLV in frozen semen samples by PCR assay
Dus Santos, M.J.; Rodriguez, Sabrina; Wigdorovitz, A. et al.
2007In AIDS Research and Human Retroviruses, 23 (4), p. 651
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Abstract :
[en] Detection of BLV in frozen semen samples by PCR assay Dus Santos Maria Jose; Rodriguez Sabrina; Wigdorovitz Andrés; Trono Karina Instituto de Virología, CICVyA, INTA-Castelar. CC 77. Buenos Aires, Argentina. Detection of BLV in frozen semen samples by PCR assay María José Dus Santos, Sabrina Rodriguez, Andrés Wigdorovitz and Karina Trono. Instituto de Virología, CICVyA, INTA-Castelar. CC 77. Buenos Aires, Argentina mdussantos@cnia.inta.gov.ar The sanitary and economic impact of BLV infection is associated with the interference in the international movement of cattle and their germ plasm. Although experimental data support the improbability that semen from BLV-positive bulls could infect recipient cows, restriction for commercialization of semen from infected animals is still present. The objective of this work was to standardize a PCR assay to diagnose the presence of BLV genome and to describe the pattern of BLV detection in frozen semen samples. The developed methodology involves the amplification of an internal fragment of gag gene and posses a limit of detection of 60 viral particles. Additionally, a biological test in susceptible sheep was performed in order to evaluate the transmission of BLV genome by semen from seropositive animals. This data strongly suggest that semen from seropositive bulls that resulted negative by PCR can be used for artificial insemination (AI), accompanied by proper collection protocols. Frozen semen samples from 30 seropositive bulls were analyzed. It was possible to detect proviral DNA in 118 out of 545 samples. It was important to note that BLV genome detection occurred in several collections but in an alternated way with non detection periods. On the other hand, in 4 seropositive bulls, it was not possible to detect BLV genome in all the samples analyzed. The development of this PCR assay constitutes a valuable diagnostic tool to determine the BLV-free status of semen used for AI. Moreover, the results suggest that BLV could present an intermittent pattern of excretion.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Dus Santos, M.J.
Rodriguez, Sabrina ;  Université de Liège - ULiège > Chimie et bio-industries > Biologie cell. et moléc.
Wigdorovitz, A.
Trono, K.
Language :
English
Title :
Detection of BLV in frozen semen samples by PCR assay
Publication date :
23 April 2007
Event name :
13th International Conference on Human Retrovirology: HTLV and Related Viruses.
Event organizer :
International Retrovirology Association
Event place :
Hakone, Japan
Event date :
from 22-05-2007 to 25-05-2007.
Audience :
International
Journal title :
AIDS Research and Human Retroviruses
ISSN :
0889-2229
eISSN :
1931-8405
Publisher :
Mary Ann Liebert, Inc., Larchmont, United States - New York
Volume :
23
Issue :
4
Pages :
651
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 30 January 2013

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