Reference : Detection of bovine leukemia virus specific antibodies using recombinant p24-ELISA.
Scientific journals : Article
Life sciences : Veterinary medicine & animal health
Detection of bovine leukemia virus specific antibodies using recombinant p24-ELISA.
Gutierrez, G. [> >]
Alvarez, I. [> >]
Fondevila, N. [> >]
Politzki, R. [> >]
Lomonaco, M. [> >]
Rodriguez, Sabrina [National Institute of Agriculture Technology INTA-Castelar, Argentina > CNIA > Institute of Virology > >]
Dus Santos, M. J. [> >]
Trono, K. [> >]
Veterinary Microbiology
Yes (verified by ORBi)
[en] Animals ; Antibodies, Viral/blood ; Cattle ; Cloning, Molecular ; Enzootic Bovine Leukosis/blood/diagnosis/immunology ; Enzyme-Linked Immunosorbent Assay/veterinary ; Escherichia coli/metabolism ; Gene Expression Regulation ; Leukemia Virus, Bovine/immunology ; Recombinant Proteins ; Viral Core Proteins/immunology
[en] We developed an indirect ELISA test (rp24-ELISA) for the detection of antibodies against the p24 capsid protein of bovine leukemia virus (BLV) in bovine serum samples. A recombinant p24 (rp24) was expressed in the Escherichia coli expression system, purified in a nickel charged resin and used as antigen in the ELISA test. A cut-off point was established considering the distribution of reactivity values obtained by rp24-ELISA on a set of 555 field serum samples that were stated as double positive (DP) or double negative (DN) by the combination of commercial agar gel immunodiffusion (AGID) assay and gp51-ELISA tests results. The rp24-ELISA showed good concordance with the agar gel immunodiffusion assay, when 710 serum samples were analyzed. In addition, rp24-ELISA demonstrated to be a precise assay with good repeatability and reproducibility and a better analytical sensitivity than AGID. From 67 discordant rp24-ELISA-AGID sera, 4 negative reactors were from the DP group, 28 positive samples were from the DN group and 35 positive samples were stated as negative by AGID but positive by the gp51-ELISA. Samples from this last subgroup were sent to an OIE reference laboratory where 28 samples were stated as negative, 5 samples were stated as positive and 2 as inconclusive. Complete concordance with blind previous results from an international proficiency test panel confirmed the capability of the assay to discriminate between infected and non-infected animals. In conclusion, the rp24-ELISA developed and standardized demonstrated to have good analytical characteristics to be considered for screening of BLV.

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