[en] Concentrations of three human liver microsomal cytochrome P-450 isozymes and 20 different monooxygenase activities were determined in human liver microsomal preparations. The results of correlation analysis suggest that: there are important variations in the amounts of the three cytochrome P-450 isozymes measured, particularly P-450(8) and P-450(9); aldrin epoxidase, d-benzphetamine N-demethylase, and S-warfarin 4-hydroxylase activities are linked to cytochrome P-450(5); aryl hydrocarbon (benzo(a)pyrene) hydroxylase and 4-nitroanisole-O-demethylase activities are linked to P-450(8); hydroxylations at the 4'-, 6-, 7-, and 8-positions of R-warfarin are closely linked to each other but are not correlated with other measured monooxygenase activities or P-450 isozyme levels; and P-450(9) is not related to any of the catalytic activities tested. Thus, certain monooxygenase activities can be attributed to specific cytochrome P-450 isozymes. This approach should be useful in suggesting the roles of different cytochromes P-450 in drug metabolism in man which can be further examined using in vitro and in vivo methods.
Disciplines :
General & internal medicine
Author, co-author :
Beaune, P H
Kremers, P G
Kaminsky, L S
De Graeve, Jean ; Université de Liège - ULiège > Services généraux (Faculté de médecine) > Relations académiques et scientifiques (Médecine)
Albert, Adelin ; Université de Liège - ULiège > Département des sciences de la santé publique > Informatique médicale et biostatistique
Guengerich, F P
Language :
English
Title :
Comparison of monooxygenase activities and cytochrome P-450 isozyme concentrations in human liver microsomes.
Publication date :
1986
Journal title :
Drug Metabolism and Disposition: the Biological Fate of Chemicals
ISSN :
0090-9556
eISSN :
1521-009X
Publisher :
Williams and Wilkins, Baltimore, United States - Maryland
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