[en] The effect of propofol on the toxicity induced by glutamate (GLU), N-methyl-D-aspartate (NMDA), kainate (KA), and amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) was investigated on cultured fetal rat hippocampal neurons. The degree of neuronal injury was quantified by measuring the release of the neuron-specific enolase (NSE) into the culture media. The toxicity induced by brief exposure to GLU (10(-4) M) or to NMDA (10(-4) M) was significantly reduced by propofol, whereas that elicited by KA, AMPA (10(-4) M), or long GLU exposure was unaffected. In conclusion, high concentrations of propofol significantly attenuate NMDA receptor-mediated glutamate neurotoxicity in vitro. Further studies are needed to confirm this beneficial effect in vivo and to evaluate propofol as a neuroprotective anesthetic agent in pathologies involving glutamate release and NMDA-mediated toxicity.
Disciplines :
General & internal medicine
Author, co-author :
Hans, Pol ; Université de Liège - ULiège > Département des sciences cliniques > Anesthésie et réanimation
Bonhomme, Vincent ; Université de Liège - ULiège > Département des sciences cliniques > Département des sciences cliniques
Collette, Julien ; Centre Hospitalier Universitaire de Liège - CHU > Chimie médicale
Albert, Adelin ; Université de Liège - ULiège > Département des sciences de la santé publique > Informatique médicale et biostatistique
Moonen, Gustave ; Université de Liège - ULiège > Département des sciences cliniques > Neurologie - Doyen de la Faculté de Médecine
Language :
English
Title :
Propofol Protects Cultured Rat Hippocampal Neurons against N-Methyl-D-Aspartate Receptor-Mediated Glutamate Toxicity
Publication date :
October 1994
Journal title :
Journal of Neurosurgical Anesthesiology
ISSN :
0898-4921
eISSN :
1537-1921
Publisher :
Lippincott Williams & Wilkins, United States - Maryland