Unpublished conference/Abstract (Scientific congresses and symposiums)
Genetic variation within β3 subunit integrin sequences and rodent species susceptibility to pathogenic hantaviruses
Pagès, Marie; Tatard, Caroline; Galan, Maxime et al.
20104th International Conference on Rodent Biology and Management
 

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Keywords :
: Murinae, Hantavirus, Seoul virus, integrin receptor, epidemiology, reservoir
Abstract :
[en] Recent investigations in rodent systematics and epidemiology have led to the discovery of numerous Hantavirus species in Asia. As seroprevalences are very low, the screening of thousands of rodents is required to genetically identify these hantaviruses and to determine their human pathogenesis as well as their rodent reservoirs. Pathogenic and non-pathogenic hantaviruses use different integrin receptors to enter cells. In particular, human integrins αIIaβ3 and αvβ3 can mediate cellular entry of hemorragic fever with renal syndrome (HFRS)- and hemorragic pulmonary syndrome (HPS)-causing hantaviruses. In contrast, non-pathogenic or low pathogenic hantaviruses were demonstrated to enter the cell via integrin αvβ1. We tested the hypothesis that amino-acid variation in β3 subunit integrin sequences could provide keys to determine a priori the possibility for a rodent species to carry a human pathogenic hantavirus. We sequenced 330 bp of the β3 chain integrin encompassing the Plexin-Semaphorin-Integrin domain, for 63 individuals corresponding to 14 Asian rodent species. We found 65 variable sites and a high level of divergence between species (up to 12%). Although the genetic variation reflected the neutral phylogeny of these species, we found one site differing between rodent species carrying pathogenic hantaviruses and non reservoir rodent species. We also detected another variable site that could be under positive selection, but only in rodent species that do not carry pathogenic hantaviruses. This site did not exhibit any genetic variation in rodent species that carry hantaviruses. In consequence, it is likely that knowing the amino-acid present at this position for a given rodent species could help us determining a priori whether this species is a reservoir of pathogenic hantaviruses. Further investigations are required to examine whether amino-acid changes at these residues could mediate conformational changes that would prevent the fixation of pathogenic hantaviruses.
Disciplines :
Genetics & genetic processes
Author, co-author :
Pagès, Marie ;  Centre de Biologie et de Gestion et des Populations (CBGP) > INRA
Tatard, Caroline;  Centre de Biologie et de Gestion et des Populations (CBGP) > INRA
Galan, Maxime;  Centre de Biologie et de Gestion et des Populations (CBGP) > INRA
Kakko, Caroline;  Centre de Biologie et de Gestion et des Populations (CBGP) > INRA
Chaval, Yannick;  Centre de Biologie et de Gestion et des Populations (CBGP) > INRA
Cosson, Jean-François;  Centre de Biologie et de Gestion et des Populations (CBGP) > INRA
Charbonnel, Nathalie;  Centre de Biologie et de Gestion et des Populations (CBGP) > INRA
Language :
English
Title :
Genetic variation within β3 subunit integrin sequences and rodent species susceptibility to pathogenic hantaviruses
Publication date :
April 2010
Event name :
4th International Conference on Rodent Biology and Management
Event place :
Bloemfontein, South Africa
Event date :
du 12 au 16 Avril 2010
Audience :
International
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since 18 January 2013

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