Acipenser sturio Ancient DNA Conservation genetics Species assignment Rhône River Protohistory
Abstract :
[en] Sturgeon disappeared from the Rhoˆne River in the mid 70’s without certitude about which species it was and about the existence of a sympatry between European sturgeon, Acipenser sturio, and Adriatic sturgeon, A. naccarii, in this watershed. In order to reach a reliable specific determination of this extinct sturgeon population, archaeozoological remains of the Jardin d’Hiver in Arles city, on the Rhoˆne river banks, were genetically analysed, following strict criteria of authentication for the ancient DNA work. The rich collection of Arles sturgeon bone remains stems from human activities between the 6th and the 2nd Century BC. Sequences of 86 bp of the cytochrome b gene were obtained on four bones, from different anatomical parts of the fish and from different archaeological layers. All gave A. sturio diagnostic sequences. This preliminary analysis is an essential first step in the project of sturgeon reintroduction in the Rhoˆne River. Thus, further analyses on a larger sample are necessary to comfort this result and to solve the question of sympatry with A. naccarii.
Disciplines :
Zoology
Author, co-author :
Pagès, Marie ; Institut des Sciences de l’Evolution, UMR 5554 (UM2-CNRS), Université Montpellier 2 > et Institut de Génomique Fonctionnelle de Lyon
Desse-Berset, Nathalie; Laboratory of Archaeozoology, CEPAM/CNRS, UMR 6130, Université de Nice-Sophia Antipolis
Tougard, Christelle; Biogéosciences-Dijon, UMR 5561, Université de Bourgogne
Brosse, Laurent; AQUA-LOGIQ
Hänni, Catherine; Institut de Génomique Fonctionnelle de Lyon
Berrebi, Patrick; Institut des Sciences de l’Evolution, UMR 5554 (UM2-CNRS), Université Montpellier 2
Language :
English
Title :
Historical presence of the sturgeon Acipenser sturio in the Rhône basin determined by the analysis of ancient DNA cytochrome b sequences
A Almodovar A Machordom J Suarez 2000 Preliminary results from characterization of Iberian Peninsula sturgeon based on analysis of the mt-DNA cytochrome b Bol Inst Esp Oceanogr 16 17 27
Arcelin P (2000) Arles protohistorique, agglomération et structuration urbaine. In: Baudat M (ed) Espace et urbanisme d'Arles, des origines à nos jours. Arles, pp 7-23
U Arnason A Gullberg A Janke J Joss C Elmerot 2004 Mitogenomic analyses of deep gnathostome divergences: a fish is a fish Gene 333 61 70
Birstein VJ, Bemis WE, Waldman JR (1995) The threatened status of acipenseriform fishes: a summary. In: Birstein VJ, Bemis WE, Waldman JR (eds) Sturgeon biodiversity and conservation. Kluwer Academic Publishers, Dordrecht-Boston-London, pp 427-435
VJ Birstein WE Bemis JR Waldman 1997 The threatened status of acipenseriform species: a summary Environ Biol Fish 48 427 435
VJ Birstein P Doukakis R DeSalle 2000 Polyphyly of mtDNA lineages in the Russian sturgeon, Acipenser gueldenstaedtii: forensic and evolutionary implications Conserv Genet 1 81 88
JR Brown TL Gilbert DJ Kowbel PJ O'Hara NE Buroker AT Beckenbach MJ Smith 1989 Nucleotide sequence of the apocytochrome B gene in white sturgeon mitochondrial DNA Nucleic Acids Res 17 43 89
R De la Herran F Robles JA Lorente C Ruiz Rejon MA Garrido-Ramos M Ruiz Rejon 2004 Genetic identification of western Mediterranean sturgeons and its implication for conservation Conserv Genet 5 545 551
N Desse-Berset 1994 Sturgeons of the Rhône during Protohistory in Arles (6th-2nd century BC). In: Fish Exploitation in the Past. Proceedings of the 7th Meeting of the ICAZ Fish Remains Working Group (Louvain, Sept. 1993) Ann Mus Roy Afr Centr Tervueren 274 81 90
P Doukakis VJ Birstein R DeSalle AN Ludwig A Ludwig A Machordom A Almodovar B Elvira 2000 Failure to confirm previous identification of two putative museum specimens of the Atlantic sturgeon, Acipenser sturio, as the Adriatic sturgeon, A. naccarii Mar Biol 136 373 377
MA Dugo BR Kreiser ST Ross WT Slack RJ Heise BR Bowen 2004 Conservation and management implications of fine-scale genetic structure of Gulf sturgeon in the Pascagoula River, Mississippi J Appl Ichthyol 20 243 251
B Elvira A Almodovar 1999 A morphological study of native sturgeon Acipenser sturio in Spain, and recent records of exotic Siberian sturgeon A. baerii J Appl Ichthyol 15 278 279
N Galtier M Gouy C Gautier 1996 SEAVIEW and PHYLO-WIN: two graphic tools for sequence alignment and molecular phylogeny Comp Appl Biosci 12 543 548
MA Garrido-Ramos MC Soriguer R De la Herran M Jamilena C Ruiz-Rejon A Domezain JA Hernando M Ruiz-Rejon 1997 Morphometric and genetic analysis as proof for the existence of two sturgeon species in the Guadalquivir river Mar Biol 129 33 39
MT Gilbert AJ Hansen E Willerslev L Rudbeck I Barnes N Lynnerup A Cooper 2003 Characterization of genetic miscoding lesions caused by postmortem damage Am J Hum Genet 72 48 61
MT Gilbert H Bandelt M Hofreiter I Barnes 2005 Assessing ancient DNA studies Trends Ecol Evol 20 541 544
S Guindon O Gascuel 2003 A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood Syst Biol 52 696 704
S Guindon F Lethiec P Duroux O Gascuel 2005 PHYML Online-a web server for fast maximum likelihood-based phylogenetic inference Nucleic Acids Res 33 W557 559
C Hänni T Brousseau V Laudet D Stehelin 1995 Isopropanol precipitation removes PCR inhibitors from ancient bone extracts Nucleic Acids Res 23 881 882
M Hofreiter V Jaenicke D Serre A Haeseler von S Pääbo 2001 DNA sequences from multiple amplifications reveal artifacts induced by cytosine deamination in ancient DNA Nucleic Acids Res 29 4793 4799
Holcik J, Kinzelbach R, Sokolov LI, Vassilev VP (1989) General introduction to fishes acipenseriformes. In: Holcik J, Kinzelbach R, Sokolov LI, Vassilev VP (eds) The freshwater fishes of Europe. Aula Verlag, Wiesbaden, pp 367-394
I Jenneckens JN Meyer L Debus C Pitra A Ludwig 2001 Evidence of mitochondrial DNA clones of Siberian sturgeon, Acipenser baerii, within Russian sturgeon, Acipenser gueldenstaedtii, caught in the River Volga Ecol Lett 3 503 508
O Loreille L Orlando M Patou-Mathis M Philippe P Taberlet C Hänni 2001 Ancient DNA analysis reveals divergence of the cave bear, Ursus spelaeus, and brown bear, Ursus arctos, lineages Curr Biol 11 200 203
A Ludwig 2006 A sturgeon view on conservation genetics Eur J Wildl Res 52 3 8
A Ludwig F Kirschbaum 1998 Comparison of mitochondrial DNA sequences between the European and the Adriatic sturgeon J Fish Biol 52 1289 1291
A Ludwig B May L Debus I Jenneckens 2000 Heteroplasmy in the mtDNA control region of sturgeon (Acipenser, Huso and Scaphirhynchus) Genetics 156 1933 1947
JA Nylander F Ronquist JP Huelsenbeck JL Nieves-Aldrey 2004 Bayesian phylogenetic analysis of combined data Syst Biol 53 47 67
Z Peng A Ludwig D Wang R Diogo Q Wei S He 2006 Age and biogeography of major clades in sturgeons and paddlefishes (Pisces: Acipenseriformes) Mol Phylogenet Evol 42 854 862
Rambaut A, Drummond A (2003) http://evolve.zoo.ox.ac.uk/software.html
E Rochard G Castelnaud M Lepage 1990 Sturgeon (Pisces: Acipenseridae); threats and prospects J Fish Biol 37 123 132
F Ronquist JP Huelsenbeck 2003 MrBayes 3: Bayesian phylogenetic inference under mixed models Bioinformatics 19 1572 1574
H Rosenthal 2003 World sturgeon conservation society E.V. (WSCS)-An international forum for scientific discussion on and concerted actions for the effective conservation and management of the highly endangered sturgeon species J Appl Ichthyol 19 343 344
Swofford DL (2002) PAUP*. Phylogenetic Analysis using Parsimony (*and other methods). Version 4.0b10. Sinauer Associates, Sunderland, Massachusetts
Tabardel M (1994) Le point sur la situation de l'esturgeon (Acipenser sturio L.) en Méditerranée occidentale et possibilités de réintroduction dans le Rhône. Dissertation, E.N.S.A. Rennes
Télétchéa F, Bernillon J, Duffraisse M, Laudet V, Hänni C (2008) Molecular identification of vertebrate species by oligonucleotide microarray in food and forensic samples. J Applied Ecol. doi: 10.1111/j.1365-2664.2007.01415.x
P Williot E Rochard G Castelnaud T. Rouault R Brun M Lepage P Elie 1997 Biological characteristics of European Atlantic sturgeon, Acipenser sturio, as the basis for a restoration program in France Environ Biol Fish 48 359 372
DY Yang B Eng JS Waye JC Dudar SR Saunders 1998 Technical note: improved DNA extraction from ancient bones using silica-based spin columns Am J Phy Anthropol 105 539 543
