[en] ABSTRACT: The objective of the current study was to investigate (i) the outcome of experimentally induced Escherichia coli mastitis in primiparous cows during early lactation in relation with production of eicosanoids and inflammatory indicators, and (ii) the validity of thermography to evaluate temperature changes on udder skin surface after experimentally induced E. coli mastitis. Nine primiparous Holstein Friesian cows were inoculated 24 +/- 6 days (d) after parturition in both left quarters with E. coli P4 serotype O32:H37. Blood and milk samples were collected before and after challenge with E. coli. The infrared images were taken from the caudal view of the udder following challenge with E. coli. No relationship was detected between severity of mastitis and changes of thromboxane B2 (TXB2), leukotriene B4 (LTB4) and lipoxin A4 (LXA4). However, prostaglandin E2 (PGE2) was related to systemic disease severity during E. coli mastitis. Moreover, reduced somatic cell count (SCC), fewer circulating basophils, increased concentration of tumor necrosis factor-alpha (TNF-alpha) and higher milk sodium and lower milk potassium concentrations were related to systemic disease severity. The thermal camera was capable of detecting 2-3 degrees C temperature changes on udder skin surface of cows inoculated with E. coli. Peak of udder skin temperature occurred after peak of rectal temperature and appearance of local signs of induced E. coli mastitis. Although infrared thermography was a successful method for detecting the changes in udder skin surface temperature following intramammary challenge with E. coli, it did not show to be a promising tool for early detection of mastitis.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Pezeshki, Adel; Ghent University, B-9000 Ghent, Belgium > Department of Comparative Physiology and Biometrics, Laboratory of Genetics, Drug Quality and Registration Group
Stordeur, Philippe; Centre d'Economie Rurale, B-6900 Marloie, Belgium > Departement Biotechnologie, plateforme Infectiologie Expérimentale
Wallemacq, Hugues; Université de Liège - ULiège > Laboratory of Cellular and Molecular Physiology, GIGA-Research and Faculty of Veterinary Medicine
Schynts, Frederic; Centre d'Economie Rurale, B-6900 Marloie, Belgium > Departement Biotechnologie, plateforme Infectiologie Expérimentale
Stevens, Mieke; Ghent University, B-9000 Ghent, Belgium > Department of Comparative Physiology and Biometrics, Laboratory of Genetics, Drug Quality and Registration Group
Boutet, Philippe; Université de Liège - ULiège > Laboratory of Cellular and Molecular Physiology, GIGA-Research and Faculty of Veterinary Medicine
Peelman, Luc J.; Ghent University, B-9000 Ghent, Belgium > Department of Comparative Physiology and Biometrics, Laboratory of Genetics, Drug Quality and Registration Group
De Spiegeleer, Bart; Ghent University, B-9000 Ghent, Belgium > Department of Comparative Physiology and Biometrics, Laboratory of Genetics, Drug Quality and Registration Group
Duchateau, Luc; Ghent University, B-9000 Ghent, Belgium > Department of Comparative Physiology and Biometrics, Laboratory of Genetics, Drug Quality and Registration Group
Bureau, Fabrice ; Université de Liège - ULiège > Département de sciences fonctionnelles > GIGA-R : Biochimie et biologie moléculaire
Burvenich, Christian; Ghent University, B-9000 Ghent, Belgium > Department of Comparative Physiology and Biometrics, Laboratory of Genetics, Drug Quality and Registration Group
Language :
English
Title :
Variation of inflammatory dynamics and mediators in primiparous cows after intramammary challenge with Escherichia coli.
Severity of E. coli mastitis is mainly determined by cow factors. Burvenich C, Van Merris V, Mehrzad J, Diez-Fraile A, Duchateau L, Vet Res 2003 34 05 521 564 10.1051/vetres:2003023 14556694 (Pubitemid 37419855)
Virulence factors of Escherichia coli isolated from cows with acute mastitis. Sanchez-Carlo V, McDonald JS, Packer RA, Am J Vet Res 1984 45 1775 1777 6388441
Efficacy of chlorhexidine as a postmilking teat disinfectant for the prevention of bovine mastitis during lactation. Oliver SP, King SH, Lewis MJ, Torre PM, Matthews KR, Dowlen HH, J Dairy Sci 1990 73 2230 2235 10.3168/jds.S0022-0302(90)78903-5 2229606
An integrated view on how the management of the dry period length of lactating cows could affect mammary biology and defence. Pezeshki A, Capuco AV, De Spiegeleer B, Peelman L, Stevens M, Collier RJ, Burvenich C, J Anim Physiol Anim Nutr 2010 94 7 30 10.1111/j.1439-0396.2010.00991.x
Moderate inflammatory reaction during experimental E. coli mastitis in primiparous cows. Vangroenweghe F, Duchateau L, Burvenich C, J Dairy Sci 2004 87 886 895 10.3168/jds.S0022-0302(04)73233-6 15259223 (Pubitemid 44151714)
Preinfection in vitro chemotaxis, phagocytosis, oxidative burst, and xpression of CD11/CD18 receptors and their predictive capacity on the outcome of mastitis induced in dairy cows with Escherichia coli. van Werven T, Noordhuizen-Stassen EN, Daemen AJJM, Schukken YH, Brand A, Burvenich C, J Dairy Sci 1997 80 67 74 10.3168/jds.S0022-0302(97)75913-7 9120097 (Pubitemid 127447116)
Increased surface expression of CD11b receptors on PMN is not sufficient to sustain phagocytosis during Escherichia coli mastitis in early postpartum dairy cows. Dosogne H, Burvenich C, van Werven T, Roets E, Noordhuizen-Stassen EN, Goddeeris B, Vet Immunol Immunopathol 1997 60 47 59 10.1016/S0165-2427(97) 00089-5 9533266 (Pubitemid 28034283)
Interaction between the respiratory burst activity of neutrophil leukocytes and experimentally induced E. coli mastitis in cows. Heyneman R, Burvenich C, Vercauteren R, J Dairy Sci 1990 73 985 994 10.3168/jds.S0022- 0302(90)78756-5 2161024
Severity of experimental Escherichia coli mastitis in ketonemic and nonketonemic dairy cows. Kremer WDJ, Noordhuizen-Stassen EN, Grommers FJ, Schukken YH, Heeringa R, Brand A, J Dairy Sci 1993 76 3428 3436 10.3168/jds.S0022-0302(93)77681-X 8270685
Classification of newly calved cows into moderate and severe responders to experimentally induced Escherichia coli mastitis. Vandeputte-Van Messom G, Burvenich C, Roets E, Massart-Len A-M, Heyneman R, Kremer WDJ, Brand A, J Dairy Res 1993 60 19 29 10.1017/S002202990002731X 8436664
Lipid mediator class switching during acute inflammation: signals in resolution. Levy BD, Clish CB, Schmidt B, Gronert K, Serhan CN, Nat Immunol 2001 2 612 619 10.1038/89759 11429545 (Pubitemid 32677628)
Detection of clinical mastitis with the help of a thermal camera. Hovinen M, Siivonen J, Taponen S, Hänninen L, Pastell M, Aisla A-M, Pyörälä S, J Dairy Sci 2008 91 4592 4598 10.3168/jds.2008-1218 19038934 (Pubitemid 352847749)
Basic investigations to evaluate a highly sensitive infrared-thermograph- technique to detect udder inflammation in cows. Barth K, Milchwissenschaft 2000 55 607 609
Respiratory burst activity of blood and milk neutrophils in dairy cows during different stages of lactation. Mehrzad J, Dosogne H, Meyer E, Heyneman R, Burvenich C, J Dairy Res 2001 68 399 415 10.1017/S0022029901005039 11694043 (Pubitemid 32990204)
Arachidonic acid metabolites in milk of cows during acute mastitis. Anderson KL, Kindahl H, Petroni A, Smith AR, Gustafsson BK, Am J Vet Res 1985 46 1573 1577 3927804 (Pubitemid 15063531)
Effect of prostaglandin synthetase inhibitors on mammary blood flow during experimentally induced mastitis in lactating goats. Burvenich C, Peeters G, Arch Int Pharmacodyn Thér 1982 258 128 137 (Pubitemid 12048824)
Temporal changes in metabolites of prostanoids in milk of heifers after intramammary infusion of Escherichia coli organisms. Peter AT, Clark PW, Van Roekel DE, Luker CW, Gaines JD, Bosu WT, Prostaglandins 1990 39 451 457 10.1016/0090-6980(90)90125-F 2111572 (Pubitemid 20160014)
Endotoxin-induced bovine mastitis: arachidonic acid metabolites in milk and plasma and effects of flunixin meglumine. Anderson KL, Kindahl H, Smith AR, Davis LE, Gustafsson BK, Am J Vet Res 1986 47 1373 1377 3089083
Short-term changes of mRNA expression of various inflammatory factors and milk proteins in mammary tissue during LPS-induced mastitis. Schmitz S, Pfaffl MW, Meyer HHD, Bruckmaier RM, Domest Anim Endocrinol 2004 26 111 126 10.1016/j.domaniend.2003.09.003 14757184 (Pubitemid 38192786)
Use of infrared thermography for early detection of mastitis in dairy cows. Scott SL, Schaefer AL, Tong AKW, Lacasse P, Can J Anim Sci 2000 80 764 765
Infrared thermography as a tool to study the milking process: a review. Kunc P, Knizkova I, Prikryl M, Maloun J, Agricultura Tropica et Subtropica 2007 40 29 32
Mammary blood flow during experimental Escherichia coli endotoxin induced mastitis in goats and cows. Dhondt G, Burvenich C, Peeters G, J Dairy Res 1977 44 433 440 10.1017/S0022029900020392 338650
