Reference : Effect of omp10 or omp19 deletion on Brucella abortus outer membrane properties and v...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/138093
Effect of omp10 or omp19 deletion on Brucella abortus outer membrane properties and virulence in mice.
English
Tibor, Anne [> > > >]
Wansard, Valerie [> > > >]
Bielartz, Valery [> > > >]
Delrue, Rose-May [> > > >]
DANESE, Isabelle mailto [Université de Liège - ULiège > > GIGA-M : Coordination scientifique]
Michel, Patrick [> > > >]
Walravens, Karl [> > > >]
Godfroid, Jacques [> > > >]
Letesson, Jean-Jacques [> > > >]
2002
Infection and Immunity
70
10
5540-6
Yes (verified by ORBi)
International
0019-9567
[en] Animals ; Antigens, Bacterial ; Bacterial Outer Membrane Proteins/genetics/physiology ; Brucella abortus/genetics/growth & development/pathogenicity/physiology ; Brucellosis/etiology/microbiology ; Cattle ; Cell Line ; Female ; Gene Deletion ; Genes, Bacterial ; Hela Cells ; Humans ; Lipoproteins ; Mice ; Mice, Inbred BALB C ; Mutation
[en] The distinctive properties of Brucella outer membrane have been considered to be critical for Brucella sp. virulence. Among the outer membrane molecules possibly related to these properties, Omp10 and Omp19 are immunoreactive outer membrane lipoproteins. Moreover, these proteins of Brucella could constitute a new family of outer membrane proteins specifically encountered in the family RHIZOBIACEAE: We evaluated the impact of omp10 or omp19 deletion on Brucella abortus outer membrane properties and virulence in mice. The omp10 mutant was dramatically attenuated for survival in mice and was defective for growth in minimal medium but was not impaired in intracellular growth in vitro, nor does it display clear modification of the outer membrane properties. Significantly fewer brucellae were recovered from the spleens of mice infected with the omp19 mutant than from those of mice infected with the parent strain at 4 and 8 weeks postinfection. The omp19 mutant exhibited an increase in sensitivity to the polycation polymyxin B and to sodium deoxycholate. These results indicate that inactivation of the omp19 gene alters the outer membrane properties of B. abortus.
http://hdl.handle.net/2268/138093
10.1128/IAI.70.10.5540-5546.2002

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