[en] The objective of this study was to describe a standardised and non-invasive method for exhaled breath condensate (EBC) collection in cats and to test whether determination of hydrogen peroxide (H2O2) in EBC might be used as marker of lower airway inflammation. The technique of barometric whole body plethysmography for cats was combined with a system to condense the effluent air from the plethysmograph, allowing simultaneous EBC collection and respiratory pattern measurement. H2O2 was determined spectrophotometrically. Eighteen experimental cats were used to investigate the impact on EBC volume and EBC H2O2 of plethysmograph ventilation rate, collection duration, sample stability, within-day and day-to-day variability. After determination of a standardised EBC collection procedure, correlation analyses between EBC H2O2 and bronchoalveolar lavage (BAL) cytology of healthy and allergen-challenged Ascaris suum (AS)-sensitised cats were performed. A significant and positive correlation between EBC H2O2 and bronchoalveolar lavage (BAL) neutrophil% was found in healthy cats (P < 0.001, r = 0.55), whereas in AS-sensitised cats, correlation with BAL eosinophil% was significant (P < 0.005, r = 0.61). H2O2 was increased after an allergen challenge in AS-sensitised cats (n = 6, 0.56 ± 0.12 versus 1.08 ± 0.35 μ mol/L, P < 0.05). This study proposes a non-invasive, well tolerated and repeatable method of EBC collection for cats and suggests that EBC H2O2 might be used as non-invasive biomarker for monitoring lower airway inflammation.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Kirschvink, N.
Marlin, D.
Castermans, Françoise ; Centre Hospitalier Universitaire de Liège - CHU > Bloc opératoire NDB
Leemans, Jérôme ; Université de Liège - ULiège > Département de sciences fonctionnelles > Pharmacologie, pharmacothérapie et toxicologie
Clercx, Cécile ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Pathologie médicale des petits animaux
Sparkes, A.
Gustin, Pascal ; Université de Liège - ULiège > Département de sciences fonctionnelles > Pharmacologie, pharmacothérapie et toxicologie
Language :
English
Title :
Collection of exhaled breath condensate and analysis of hydrogen peroxide as a potential marker of lower airway inflammation in cats
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Bibliography
Antczak A. Nowak D. Shariati M. Król M. Piasecka G. Kurmanowska Z. Increased hydrogen peroxide and thiobarbituric acid-reactive products in expired breath condensate of asthmatic patients European Respiratory Journal 10 1997 1235-1241
Deaton Ch. Marlin D. Newton R. Smith K. Schroter R. Expired breath condensate hydrogen peroxide correlates with bronchoalveolar lavage neutrophilia European Respiratory Journal 22 2003 18s
Dohlman A.W. Black H.R. Royall J.A. Expired breath hydrogen peroxide is a marker of acute airway inflammation in pedriatic patients with asthma American Revue of Respiratory Disease 148 1993 955-960
Dye J.A. McKiernan B.C. Rozanski E.A. Hoffman W.E. Losonsky J.M. Homco L.D. Weisiger R.M. Kakoma I. Bronchopulmonary disease in the cat: Historical, physical, radiographic, clinicopathologic, and pulmonary functional evaluation of 24 affected and 15 healthy cats Journal of Veterinary Internal Medicine 10 1996 385-400
Gallati H. Pracht I. Peroxidase aus Meerrettich: Kinetische Studien und Optimierung der Peroxidase-Aktivitätsbestimmung mit den Substraten H2O2 und 3,3',5,5'-tetramethylbenzidin Journal of Clinical Chemistry and Clinical Biochemistry 23 1985 453-460
Gessner C. Kuhn H. Seyfarth H.-J. Pankau H. Winkler J. Schauer J. Wirtz H. Factors influencing breath condensate volume Pneumologie 55 2001 414-419
Griese M. Latzin P. Beck J. A noninvasive method to collect nasally exhaled air condensate in humans of all ages European Journal of Clinical Investigation 31 2001 915-920
Halloy, D.J., Kirschvink, N.A., Vincke, G.L., Hamoir, J.N., Delvaux, F.H., Gustin,P.G., (2004). Whole body barometric plethysmography: a screening method to investigate airway reactivity and acute lung injuries in freely moving piglets. The Veterinary Journal, in press, doi:10.1016/j.tvjl.2003.10.015
Hamelmann E. Schwarze J. Takeda A. Oshiba G. Larsen G.L. Irvin G. Gelfand E.W. Noninvasive measurement of airway responsiveness in allergic mice using barometric plethysmography American Journal of Respiratory and Critical Care Medicine 156 1997 766-775
Hirt R.A. Guetl A. Gille L. Oxidant-antioxidant balance and the role of oxidative stress in feline asthma Journal of Veterinary Internal Medicine 16 2002 387
Hirt R.A. Dederichs D. Boehler A. Hoffman A.M. Relationship of age, sex, body weight, and hematologic and respiratory variables with airway reactivity in adult cats American Journal of Veterinary Research 64 2003 26-31
Hoffman A.M. Dhupa N. Cimetti L. Airway reactivity measured by barometric whole-body plethysmography in healthy cats American Journal of Veterinary Research 60 1999 1487-1492
Horvath I. Donnelly L.E. Kiss A. Paredi P. Kharitonov S.A. Barnes P.J. Raised levels of exhaled carbon monoxide are associated with an increased expression of heme oxygenase-1 in airway macrophages in asthma: A new marker of oxidative stress Thorax 53 1998 668-672
Kharitonov S.A. Barnes P.J. Exhaled markers of pulmonary disease American Journal of Respiratory and Critical Care Medicine 163 2001 1693-1722
Kharitonov S.A. Barnes P.J. Exhaled nitric oxide, carbon monoxide, and breath condensate Lung Biology in Health and Disease Disease Markers in Exhaled Breath vol. 170 2003 199-218 Marcel Dekker
Kharitonov S.A. Barnes P.J. Exhaled nitric oxide, carbon monoxide, and breath condensate in inflammatory lung disease and response to medical treatment Lung Biology in Health and Disease Disease Markers in Exhaled Breath vol. 170 2003 307-341 Marcel Dekker
Kirschvink N. Smith N. Fiévez L. Bougnet V. Art T. Degand G. Marlin D. Roberts C. Génicot B. Lindsey P. Lekeux P. Effect of chronic airway inflammation and exercise on pulmonary and systemic antioxidant status of healthy and heaves-affected horses Equine Veterinary Journal 34 2002 563-571
Loukides S. Bouros D. Papatheodorou G. Panagou P. Siafakas M.C. The relationships among hydrogen peroxide in expired breath condensate, airway inflammation, and asthma severity Chest 121 2002 338-346
Morrow J.D. Roberts L.J. The isoprostanes: Their role as an index of oxidant stress status in human pulmonary disease American Journal of Respiratory and Critical Care Medicine 166 2002 S25-S30
Mutlu G.M. Garey K.W. Robbins R.A. Danziger L.A. Rubinstein I. Collection and analysis of exhalated breath condensate in humans American Journal of Respiratory and Critical Care Medicine 164 2001 731-737
Nowak D. Kalucka S. Bialasiewicz P. Król M. Exhalation of H2O2and thiobarbituric acid reactive substances (TBARS) by healthy subjects Free Radical Biology and Medicine 30 2001 178-186
Padrid P.A. Feldman B.F. Funk K. Samitz E.M. Reil D. Cross C.E. Cytologic, microbiologic, and biochemical analysis of bronchoalveolar lavage fluid obtained from 24 healthy cats American Journal of Veterinary Research 52 1991 1300-1307
Padrid P.A. Snook S. Finucane T. Shiue P. Cozzi P. Solway J. Leff A.R. Persistent airway hyperresponsiveness and histologic alterations after chronic antigen challenge in cats American Journal of Respiratory and Critical Care Medicine 151 1995 184-193
Padrid P.A. Feline asthma: Diagnosis and treatment Veterinary Clinics of North America: Small Animal Practice 30 2000 1279-1294
Paredi P. Kharitonov S.A. Barnes P.J. Analysis of expired air for oxidation products American Journal of Respiratory and Critical Care Medicine 166 2002 S31-S37
Reinhold P. Becher G. Rothe M. Evaluation of the measurement of leukotriene B4 concentrations in exhaled condensate as a noninvasive method for assessing mediators of inflammation in the lungs of calves American Journal of Veterinary Research 61 2000 742-749
Sandrini A. Ferreira I.M. Jardim J.R. Zamel N. Chapman K.R. Effect of nasal triamcinolone acetonide on lower airway inflammatory markers in patients with allergic rhinitis Journal of Allergy and Clinical Immunology 111 2003 313-320
Schleiss M.B. Holz O. Behnke M. Richter K. Magnussen H. Jörres R.A. The concentration of hydrogen peroxide in exhaled air depends on expiratory flow rate European Respiratory Journal 16 2000 1115-1118
Sparkes, A., Mardell, E., Deaton, Ch., Kirschvink, N., Marlin, D. Exhaled breath condensate collection in cats - description of a non-invasive technique to investigate airway disease. Journal of Feline Medicine and Surgery, in press
Van der Vliet A. Cross C.E. Oxidants, nitrosants, and the lung American Journal of Medicine 109 2000 398-421
Vass G. Huszár E. Barát E. Valyon M. Kiss D. Pénzes I. Augusztinovicz M. Horváth I. Comparison of nasal and oral inhalation during exhaled breath condensate collection American Journal of Respiratory and Critical Care Medicine 167 2002 850-855
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