Plasma concentrations of pregnancy-associated glycoproteins measured using anti-bovine PAG-2 antibodies on Day 120 of gestation predict abortion in dairy cows naturally infected with Neospora caninum.
Garcia-ispierto, I; Almeria, S; Serrano, Bet al.
2013 • In Reproduction in Domestic Animals, 48, p. 613-618
[en] The present study sought to determine: (i) the effects of Neospora caninum infection and twin pregnancy on plasma pregnancy-associated glycoprotein-2 (PAG-2) concentrations throughout pregnancy and (ii) whether plasma PAG-2 concentrations could predict abortion in N. caninum-infected cows. The study was performed on a commercial Holstein-Friesian dairy herd in northeastern Spain and the final data included those recorded in 53 non-aborting and 19 aborting animals. Blood samples were collected immediately before pregnancy diagnosis (on Days 40, 90, 120, 150, 180 and 210 post-insemination) in non-aborting cows or until the time of abortion detection in aborting cows. General lineal models (GLM) repeated measures ANOVA revealed the different behavior of PAG-1 and PAG-2, and significant effects of Neospora seropositivity, cool season and twin pregnancy on plasma PAG-2 concentrations throughout gestation (between-subject effects). In addition, based on the odds ratios, the likelihood of abortion increased in Neospora-seropositive cows (by a factor of 7.0) compared to seronegative animals and decreased in cows with a high plasma PAG-2 concentration (>4.5 ng/ml) on Day 120 of pregnancy (by a factor of 0.24), compared to the remaining cows. In conclusion, there is a relationship between plasma PAG-2 concentrations and the risk of abortion in Neosporainfected dairy cows. Thus, plasma PAG concentrations measured using anti-boPAG-2 antiserum on Day 120 of gestation could serve as an indicator of the abortion risk in N. caninum infected animals; values <4.5 ng/ml indicating a high risk of abortion in chronically infected animals.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Garcia-ispierto, I
Almeria, S
Serrano, B
Melo de Sousa, Noelita ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction
Beckers, Jean-François ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction
Lopez-Gatius, F
Language :
English
Title :
Plasma concentrations of pregnancy-associated glycoproteins measured using anti-bovine PAG-2 antibodies on Day 120 of gestation predict abortion in dairy cows naturally infected with Neospora caninum.
Publication date :
2013
Journal title :
Reproduction in Domestic Animals
ISSN :
0936-6768
eISSN :
1439-0531
Publisher :
Blackwell Publishing, Berlin, Germany
Volume :
48
Pages :
613-618
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
Spanish CICYT grants AGL2012-39830-C02-01/GAN and AGL2012-39830-C02-01/GAN.
Almería S, Ferrer D, Pabón M, Castellà J, Mañas S, 2002: Red foxes (Vulpes vulpes) are a natural intermediate host of Neospora caninum. Vet Parasitol 107, 287-294.
Anderson ML, Andrianarivo AG, Conrad PA, 2000: Neosporosis in cattle. Anim Reprod Sci 60/61, 417-431.
Ayad A, Sousa NM, Sulon J, Iguer-Ouada M, Beckers JF, 2007: Comparison of five radioimmunoassay systems for PAG measurement: ability to detect early pregnancy in cows. Reprod Domest Anim 42, 433-440.
Beckers JF, Dewulf M, Verstegen J, Wouters-Ballman P, Ectorsm F, 1988: Isolation of a bovine chorionic gonadotrophin (bCG). Theriogenology 29, 218. (Abstract).
Beckers JF, Roberts RM, Zoli AP, Ectors F, Derivaux J, 1994: Molecules of the family of aspartic proteinases in the placenta of ruminants: hormones or proteins? Bull Mem Acad R Med Belg 149, 355-367.
Bella A, Sousa NM, Lopez-Gatius F, Garcia-Ispierto I, Schoofs L, Chahiba N, Beckers JF 2011: Development of a new radioimmunoassay using antiserum against pregnancy-associated glycoprotein ii: concentrations in Holstein dairy cattle. 6th European Congress of Bovine Health and Management (ECBHM), 7-9 September, p 63. (Abstract of Conference)
De Vries A, 2006: Economic value of pregnancy in dairy cattle. J Dairy Sci 89, 3876-3885.
Dubey JP, 2003: Review of Neospora caninum and neosporosis in cattle. Korean J Parasitol 41, 1-16.
Dubey JP, Schares G, Ortega-Mora LM, 2007: Epidemiology and control of neosporosis and Neospora caninum. Clin Microbiol Rev 20, 323-367.
Garbayo JM, Green JA, Manikkam M, Beckers JF, Kiesling DO, Ealy AD, Roberts RM, 2000: Caprine pregnancy-associated glycoproteins (PAG): their cloning, expression, and evolutionary relationship to other PAG. Mol Reprod Dev 57, 311-322.
Garbayo JM, Serrano B, Lopez-Gatius F, 2008: Identification of novel pregnancy-associated glycoproteins (PAG) expressed by the peri-implantation conceptus of domestic ruminants. Anim Reprod Sci 103, 120-134.
García-Ispierto I, López-Gatius F, Almería S, Yániz J, Santolaria P, Serrano B, Bech-Sàbat G, Nogareda C, Sulon J, de Sousa NM, Beckers JF, 2009: Factors affecting plasma prolactin concentrations throughout gestation in high producing dairy cows. Domest Anim Endocrinol 36, 57-66.
García-Ispierto I, Nogareda C, Yániz JL, Almería S, Martínez-Bello D, de Sousa NM, Beckers JF, López-Gatius F, 2010: Neospora caninum and Coxiella burnetii seropositivity are related to endocrine pattern changes during gestation in lactating dairy cows. Theriogenology 74, 212-220.
Hughes AL, Green JA, Garbayo JM, Roberts RM, 2000: Adaptive diversification within a large family of recently duplicated, placentally expressed genes. Proc Natl Acad Sci USA 97, 3319-3323.
Lee JI, Kim IH, 2007: Pregnancy loss in dairy cows: the contributing factors, the effects on reproductive performance and the economic impact. J Vet Sci 8, 283-288.
Liddell S, Jenkins MC, Dubey JP, 1999: A comparative PCR assay for quantitative detection of Neospora caninum. Int J Parasitol 29, 1583-1588.
Lindsay DS, Dubey JP, 1989: Immunohistochemical diagnosis of Neospora caninum in tissue sections. Am J Vet Res 50, 1981-1983.
López-Gatius F, García-Ispierto I, 2010: Ultrasound and endocrine findings that help to assess the risk of late embryo/early foetal loss by non-Infectious cause in dairy cattle. Reprod Domest Anim 45(Suppl. 3), 15-24.
López-Gatius F, Santolaria P, Yaniz J, Rutllant J, Lopez-Bejar M, 2002: Factors affecting pregnancy loss from gestation Day 38 to 90 in lactating dairy cows from a single herd. Theriogenology 57, 1251-1261.
López-Gatius F, López-Béjar M, Murugavel K, Pabón M, Ferrer D, Almería S, 2004a: Neospora-associated abortion episode over a 1-year period in a dairy herd in north-east Spain. J Vet Med B Infect Dis Vet Public Health 51, 348-352.
López-Gatius F, Pabón M, Almería S, 2004b: Neospora caninum infection does not affect early pregnancy in dairy cattle. Theriogenology 62, 606-613.
López-Gatius F, Garbayo JM, Santolaria P, Yániz J, Ayad A, de Sousa NM, Beckers JF, 2007a: Milk production correlates negatively with plasma concentrations of pregnancy-associated glycoprotein (PAG) during the early fetal period in high producing dairy cows with live fetuses. Domest Anim Endocrinol 32, 29-42.
López-Gatius F, Garbayo JM, Santolaria P, Yániz JL, Almería S, Ayad A, de Sousa NM, Beckers JF, 2007b: Plasma pregnancy-associated glycoprotein-1 (PAG-1) concentrations during gestation in Neospora-infected dairy cows. Theriogenology 67, 502-508.
National Research Council, 2001: Nutrient Requirements of Dairy Cattle (7th rev. ed.). National Academy Science, Washington, DC.
Pabón M, López-Gatius F, García-Ispierto I, Bech-Sàbat G, Nogareda C, Almería S, 2007: Chronic Neospora caninum infection and repeat abortion in dairy cows: a 3-year study. Vet Parasitol 147, 40-46.
Perényi Z, Szenci O, Sulon J, Drion PV, Beckers JF, 2002: Comparison of the ability of three radioimmunoassay to detect pregnancy-associated glycoproteins in bovine plasma. Reprod Domest Anim 37, 100-104.
Peters AR, 1996: Embryo mortality in the cow. Anim. Breed. Abstr. 64, 587-598.
Rodbard D, 1974: Statistical quality control and routine data processing for radioimmunoassays and immunoradiometric assays. Clin Chem 20, 1255-1270.
Serrano B, López-Gatius F, Santolaria P, Almería S, García-Ispierto I, Bech-Sabat G, Sulon J, de Sousa NM, Beckers JF, Yániz JL, 2009: Factors affecting plasma pregnancy-associated glycoprotein 1 concentrations throughout gestation in high-producing dairy cows. Reprod Domest Anim 44, 600-605.
Sousa NM, Beckers JF, 2007: Plasma pregnancy-associated glycoprotein-1 (PAG-1) concentrations during gestation in Neospora-infected dairy cows. Theriogenology, 67, 502-508.
Sousa NM, Ayad A, Beckers JF, Gajewski Z, 2006: Pregnancy-associated glycoproteins (PAG) as pregnancy markers in the ruminants. J Physiol Pharmacol 57, 153-171.
Telugu BPVL, Walker AM, Green JA, 2009: Characterization of the bovine pregnancy-associated glycoprotein gene family - analysis of gene sequences, regulatory regions within the promoter and expression of selected genes. BMC Genomics 10, 185.
Thorell JI, Johansson BG, 1971: Enzymatic iodination of polypeptides with 125I to high specific activity. Biochim Biophys Acta 251, 363-369.
Thurmond MC, Picanso JP, 1990: A surveillance system for bovine abortion. Prev Vet Med 8, 41-53.
Vaitukaitis J, Robbins JB, Nieschlag E, Ross GT, 1971: A method for producing specific antisera with small doses of immunogen. J Clin Endocrinol Metab 33, 988-991.
Vanroose G, de Kruif A, Van Soom A, 2000: Embryonic mortality and embryo-pathogen interactions. Anim Reprod Sci, 60-61, 131-143.
Xie S, Low BG, Nagel RJ, Kramer KK, Anthony RV, Zoli AP, Beckers JF, Roberts RM, 1991: Identification of the major pregnancy-specific antigens of cattle and sheep as inactive members of the aspartic proteinase family. Proc Natl Acad Sci USA 88, 10247-10251.
Xie S, Low BG, Nagel RJ, Beckers JF, Roberts RM, 1994: A novel glycoprotein of the aspartic proteinase gene family expressed in bovine placental trophectoderm. Biol Reprod 51, 1145-1153.
Zoli AP, Beckers JF, Wouters-Ballman P, Closset J, Falmagne P, Ectors F, 1991: Purification and characterization of a bovine pregnancy-associated glycoprotein. Biol Reprod 45, 1-10.
Zoli AP, Guibault LA, Delahaut P, Benitez Ortiz W, Beckers JF, 1992: Radioimmunoassay of a bovine pregnancy-associated glycoprotein in serum: its application for pregnancy diagnosis. Biol Reprod 46, 83-92.
