Article (Scientific journals)
Blastocyst transfer after aseptic vitrification of zygotes: an approach to overcome an impaired uterine environment
Vanderzwalmen, Pierre; Zech, Nicolas; Ectors, Fabien et al.
2012In Reproductive Biomedicine Online, 25, p. 591-599
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Keywords :
aseptic vitrification; blastocyst transfer; cryopreservation; 2PN; uterine receptivity; zygotes
Abstract :
[en] In some IVF cycles, no fresh embryo transfer in the stimulated cycle is advisable. The cryopreservation of zygotes and the transfer of blastocysts in a cryo-embryo transfer is an option to circumvent an inadequate uterine environment due to risk of ovarian hyperstimulation syndrome, inappropriate endometrium build up, endometrial polyps or uterine myomas. For this strategy, highly secure and safe cryopreservation protocols are advisable. This study describes a protocol for aseptic vitrification of zygotes that results in high survival rates and minimizes the potential risk of contamination in liquid nitrogen during cooling and long-term storage. In mouse zygotes, there was no difference in efficiency as compared with a conventional open vitrification system. In IVF patients, aseptically vitrified zygotes showed no difference in blastocyst formation rate as compared with sibling zygotes kept in fresh culture. A clinical study comprising 173 cryo-cycles with a transfer of blastocysts originating from vitrified zygotes showed an ongoing pregnancy rate of 40.9%. The live birth rate per patient was 36.8%. A combination of good clinical results and increased safety conditions due to aseptic vitrification encourages the use of cryo-embryo transfer for patients with a suboptimal uterine environment in a fresh cycle.
Research center :
Giga-Development and Stem Cells - ULiège
Disciplines :
Reproductive medicine (gynecology, andrology, obstetrics)
Author, co-author :
Vanderzwalmen, Pierre ;  Université de Liège - ULiège > Doct. sc. vété. (Bologne)
Zech, Nicolas
Ectors, Fabien  ;  Université de Liège - ULiège > GIGA-Management : Plate-forme transgénique
Stecher, Astrid
Lejeune, Bernard
Vanderzwalmen, Sabine
Wirleitner, Barbara
Language :
English
Title :
Blastocyst transfer after aseptic vitrification of zygotes: an approach to overcome an impaired uterine environment
Publication date :
December 2012
Journal title :
Reproductive Biomedicine Online
ISSN :
1472-6483
eISSN :
1472-6491
Publisher :
Reproductive BioMedicine Online, Cambridge, United Kingdom
Volume :
25
Pages :
591-599
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
Available on ORBi :
since 20 December 2012

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