[en] Mitochondria are key organelles in primary metabolism: they are the main source of energy production and the starting point of important biosynthetic pathways. Thus the control of the mitochondrial function is a prerequisite for cellular survival in front of a wide spectrum of exogenous or endogenous stresses. By using the SILAC method, we have investigated the yeast mitochondrial and cellular proteome adaptation to the heterologous expression of a mitochondrial alternative oxidase (AOX). AOX is an energy dissipating enzyme that catalyses the re-oxidation of ubiquinol within the respiratory chain and compete with the Cytochrome pathway for electrons and thus, prevents proton pumping, and consequently leads to a decrease in ATP synthesis.
