Article (Scientific journals)
Necrotoxigenic Escherichia Coli Type-2 Invade and Cause Diarrhoea During Experimental Infection in Colostrum-Restricted Newborn Calves
Van Bost, Sigrid; Roels, S.; Mainil, Jacques
2001In Veterinary Microbiology, 81 (4), p. 315-29
Peer Reviewed verified by ORBi
 

Files


Full Text
VanBost_NTEC2.pdf
Publisher postprint (255.74 kB)
Download

All documents in ORBi are protected by a user license.

Send to



Details



Abstract :
[en] There exists experimental evidence that necrotoxigenic Escherichia coli (NTEC) strains producing the cytotoxic necrotising factor 1 cause intestinal and extra-intestinal disease in piglets. On the other hand, no experimental model has been developed with NTEC strains producing the cytotoxic necrotising factor 2. In all, 14 colostrum-restricted calves were orally challenged with two strains isolated from the faeces of a diarrheic calf (B20a) or from the heart blood of a septicaemic calf (1404). All calves had diarrhoea which lasted until euthanasia in eight of them. In those calves, diarrhoea was correlated with the faecal excretion of the challenge strains. At necropsy, vascular congestion of the intestinal mucosa, hypertrophy of the mesenteric lymph nodes (MLN) and some congestion of the lungs were observed. Bacteriology confirmed the colonisation of the intestine by the challenge strains which were also recovered from the heart blood, the lungs and/or the liver. Histological sections confirmed enterocolitis, lymphadenitis and limited bronchopneumonia. In the intestinal tissue sections, bacteria testing positive in an in situ DNA hybridisation assay with a CNF2 probe were observed. Those results were confirmed by immunohistochemistry with a polyclonal anti-O78 and a monoclonal anti-F17b antisera. Three of the five control calves receiving either saline or a CNF(-), F17a strain (25KH09) had no clinical signs or lesions. The other two presented a profuse liquid diarrhoea but those calves were positive for the presence of K99(+) E. coli. In this model, both NTEC2 strains were thus, able to colonise the intestine, to cause long-lasting diarrhoea and to invade the blood stream with localisation in various internal organs in colostrum-restricted conventional newborn calves.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Van Bost, Sigrid
Roels, S.
Mainil, Jacques ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Bactériologie et pathologie des maladies bactériennes
Language :
English
Title :
Necrotoxigenic Escherichia Coli Type-2 Invade and Cause Diarrhoea During Experimental Infection in Colostrum-Restricted Newborn Calves
Publication date :
20 August 2001
Journal title :
Veterinary Microbiology
ISSN :
0378-1135
eISSN :
1873-2542
Publisher :
Elsevier, Netherlands
Volume :
81
Issue :
4
Pages :
315-29
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 21 November 2012

Statistics


Number of views
53 (0 by ULiège)
Number of downloads
249 (1 by ULiège)

Scopus citations®
 
24
Scopus citations®
without self-citations
19
OpenCitations
 
17

Bibliography


Similar publications



Contact ORBi