Reference : Measurement of serum albumin by protein electrophoresis and bromocresol green methods...
Scientific congresses and symposiums : Paper published in a journal
Life sciences : Veterinary medicine & animal health
Measurement of serum albumin by protein electrophoresis and bromocresol green methods in canine and equine patients
Ramery, Eve mailto [Université de Liège - ULiège > Département de sciences fonctionnelles > Biochimie >]
Bureau, Fabrice mailto [Université de Liège - ULiège > Département de sciences fonctionnelles > GIGA-R : Biochimie et biologie moléculaire >]
Veterinary Clinical Pathology
American Society for Veterinary Clinical Pathology
Yes (verified by ORBi)
Baton Rouge
15th Biennial Congress of the International Society for Animal Clinical Pathology & 14th Conference of the European Society of Veterinary Clinical Pathology
du 02 au 09 Juillet 2012
[en] Albumin ; electrophoresis ; bromocresol green
[en] Background: Bromocresol green method (BCG) is routinely used in veterinary laboratories to measure albumin. A good agreement between BCG and electrophoresis (SPE) in healthy domestic animals is reported. However, in human medicine, at low albumin and high globulin concentrations, BCG also binds to α- and β-globulins fractions. Surprisingly, there is no recent literature evaluating the BCG method in diseased domestic animals. Objective: The purpose of the present study was therefore to compare the serum albumin values obtained by SPE and BCG in canine and equine patients. Methods: Albumin measured by BCG and SPE was compared in the serum from 30 canine and 30 equine patients. Results: Good correlation was found (Spearman´s rho rs = 0.91 in dogs and 0.84 in horses). However, Altman and Bland analysis of results showed a positive bias of BCG with SPE (3.41 g/l in horses and 0.41 g/l in dogs) that increased when only hypoalbuminemic patients were considered (4.17 g/l in horses and 1.31 g/l in dogs). Conclusion: Results confirm that, despite good correlation, BCG method tends to overestimate albumin, especially in hypoalbuminemic patients. This is particularly evident in horses.
Researchers ; Professionals

File(s) associated to this reference

Fulltext file(s):

Restricted access
esvcp2012.pdfAuthor preprint178.29 kBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.