Article (Scientific journals)
Endosulfan effects on Rana dalmatina tadpoles: Quantitative developmental and behavioural analysis
Lavorato, Manuela; Bernabo, Ilaria; Crescente, Antonio et al.
2013In Archives of Environmental Contamination and Toxicology, 64 (2), p. 253-262
Peer Reviewed verified by ORBi
 

Files


Full Text
Arch_Environ_Contam_Toxicol_2013-Author-postprint.pdf
Author postprint (475 kB)
This is the pdf of the author postprint (i.e. post-reviewed version) in open access
Download
Full Text Parts
Arch_Environ_Contam_Toxicol_2013.pdf
Publisher postprint (725.07 kB)
This is the published pdf
Request a copy

This paper is published by Springer and available at http://link.springer.com


All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
Ecotoxicology; Pesticide; Organochlorine pesticide; Endosulfan; POP; Persistent organic pollutant; Amphibian; Frog; Tadpole; Survival; Metamorphosis; Development; Growth; Behaviour; Behavior; Video-tracking; Ethovision; Movement; Malformation; Biomarker
Abstract :
[en] Endosulfan is an organochlorine pesticide that was recently labeled as a persistent organic pollutant, but it is still widely employed, particularly in developing countries. The goal of this study is to evaluate the acute (LC50) and chronic effects (developmental and behavioural traits) of this insecticide on Rana dalmatina tadpoles after exposure to ecologically relevant concentrations (0.005, 0.01, and 0.05 mg/L) by applying video-tracking techniques to evaluate the quantitative effect of endosulfan on amphibian behavioural patterns. The 96 h LC50 value was 0.074 mg endosulfan/L. Tadpoles chronically exposed to 0.01 and 0.05 mg endosulfan/L underwent high mortality rate, decreased larval growth, delayed development, and increased incidence of malformations, and they did not reach metamorphosis by the end of the experiment. Moreover, tadpoles exposed to these concentrations exhibited several abnormalities in swimming patterns, such as shorter distance moved, swirling, resting, and unusual use of space. The exposure to 0.005 mg endosulfan/L did not cause any significant effects on behaviour, larval growth, or development, but we observed a significant decrease in both survival and time to metamorphosis. We showed that developmental abnormalities are dose-dependent and that the pesticide effects could differ depending on the endosulfan concentration and the species tested. We also validated the hypothesis that behavioural analysis, along with the use of new analytical methods, could be a useful tool in amphibian ecotoxicological studies.
Research Center/Unit :
AFFISH-RC - Applied and Fundamental FISH Research Center - ULiège
Disciplines :
Environmental sciences & ecology
Aquatic sciences & oceanology
Author, co-author :
Lavorato, Manuela;  Université de Liège - ULiège & University of Calabria > Unité de Biologie du Comportement & Department of Ecology
Bernabo, Ilaria;  University of Calabria
Crescente, Antonio;  University of Calabria
Denoël, Mathieu  ;  Université de Liège - ULiège > Département de Biologie, Ecologie et Evolution > Biologie du comportement - Ethologie et psychologie animale
Tripepi, Sandro;  University of Calabria
Brunelli, Elvira;  University of Calabria
Language :
English
Title :
Endosulfan effects on Rana dalmatina tadpoles: Quantitative developmental and behavioural analysis
Publication date :
2013
Journal title :
Archives of Environmental Contamination and Toxicology
ISSN :
0090-4341
eISSN :
1432-0703
Publisher :
Springer Science & Business Media B.V., New York, United States - New York
Volume :
64
Issue :
2
Pages :
253-262
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique
FRFC - Fonds de la Recherche Fondamentale Collective
Available on ORBi :
since 15 November 2012

Statistics


Number of views
188 (23 by ULiège)
Number of downloads
448 (3 by ULiège)

Scopus citations®
 
29
Scopus citations®
without self-citations
25
OpenCitations
 
25
OpenAlex citations
 
40

Bibliography


Similar publications



Contact ORBi