[en] Bone marrow stromal cells are adult multipotent cells that represent an attractive tool in cellular therapy strategies. Several
studies have reported that in vitro passaging of mesenchymal stem cells alters the functional and biological properties of
those cells, leading to the accumulation of genetic aberrations. Recent studies described bone marrow stromal cells (BMSC)
as mixed populations of cells including mesenchymal (MSC) and neural crest stem cells (NCSC). Here, we report the
transformation of NCSC into tumorigenic cells, after in vitro long-term passaging. Indeed, the characterization of 6 neural
crest-derived clones revealed the presence of one tumorigenic clone. Transcriptomic analyses of this clone highlighted,
among others, numerous cell cycle checkpoint modifications and chromosome 11q down-regulation (suggesting a deletion
of chromosome 11q) compared with the other clones. Moreover, unsupervised analysis such as a dendrogram generated
after agglomerative hierarchical clustering comparing several transcriptomic data showed important similarities between
the tumorigenic neural crest-derived clone and mammary tumor cell lines. Altogether, it appeared that NCSC isolated from
adult bone marrow represents a potential danger for cellular therapy, and consequently, we recommend that phenotypic,
functional and genetic assays should be performed on bone marrow mesenchymal and neural crest stem cells before in vivo
use, to demonstrate whether their biological properties, after ex vivo expansion, remain suitable for clinical application.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Wislet, Sabine ✱; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, et biochimie humaine
Poulet, Christophe ✱; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > GIGA-R : Génétique humaine
Neirinckx, Virginie ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie
Bours, Vincent ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > GIGA-R : Génétique humaine
Rogister, Bernard ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, et biochimie humaine
✱ These authors have contributed equally to this work.
Language :
English
Title :
In Vivo Tumorigenesis Was Observed after Injection of In Vitro Expanded Neural Crest Stem Cells Isolated from Adult Bone Marrow
Publication date :
October 2012
Journal title :
PLoS ONE
eISSN :
1932-6203
Publisher :
Public Library of Science, San Franscisco, United States - California
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