Optimization of poly(2-(dimethylamino)ethyl methacrylate)-co-poly(ethyleneglycol)/DNA complexes designed for cell transfection

Grandfils, Christian; Emonds-Alt, J.

Article (Scientific journals)

Grandfils, Christian; Emonds-Alt, J.

2005 • In Minerva Biotecnologica, 17 (4), p. 237-243

Peer Reviewed verified by ORBi

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Keywords :

cell transfection; polycation; gene delivery; polyelectrolyte complex

Abstract :

[en] Cell transfection relies upon the delivery of genomic material within cells in order to express selected DNA sequences. Non-viral gene delivery systems are preferable over viral vectors for several reasons: biosafety, low immunogenicity, higher loading capacity, and easiness of production. Their major drawbacks actually are their limited efficiency in vivo compared to viruses, their cytotoxicity and the fact that they are rapidly cleared up from the bloodstream. The aim of our study was to better characterize the physico-chemical behavior of the polycations based on poly(2-(dimethylamino)ethyl methacrylate)-co-poly(ethyleneglycol) and to control the formulation step to produce the polyelectrolyte complexes.

Research Center/Unit :

CEIB - Centre Interfacultaire des Biomatériaux - ULiège

Disciplines :

Microbiology
Biotechnology

Author, co-author :

Grandfils, Christian ; Université de Liège - ULiège > Biochimie et physiologie générale

Emonds-Alt, J.

Language :

English

Title :

Optimization of poly(2-(dimethylamino)ethyl methacrylate)-co-poly(ethyleneglycol)/DNA complexes designed for cell transfection

Publication date :

December 2005

Journal title :

Minerva Biotecnologica

ISSN :

1120-4826

eISSN :

1827-160X

Publisher :

Edizioni Minerva Medica, Turin, Italy

Volume :

Issue :

Pages :

237-243

Peer reviewed :

Peer Reviewed verified by ORBi

Name of the research project :

Nouveaux polycations et vectorisation d’ADN dans les cellules de parois vasculaires” Convention Région Wallonne n° 14612, année 2001-2004 dans le cadre du concours Initiative 2000

Funders :

DG06

Available on ORBi :

since 03 November 2012

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