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Poster (Scientific congresses and symposiums)
EVALUATION OF SV2Alox/Cre TRANSGENIC MOUSE USING [18F]UCB-H IN IN VITRO AUTORADIOGRAPHY
Serrano Navacerrada, Maria Elisa  ; Becker, Guillaume  ; MENTEN, Catherine  et al.
2015 • International Symposium "Aging and sleep: Perks for longevity?
 

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Keywords :
Epilepsy; [18F]UCB-H; hippocampus; cre-lox technique; floxed-mice; SV2A; in vitro autoradiography
Abstract :
[en] Background: SV2A is the most studied isoform of the Synaptic Vesicle 2 proteins, which are involved in the synaptic vesicle trafficking. Interestingly, the SV2A has been identify as the binding site for the antiepileptic drug levetiracetam, showing a close relation between the epilepsy, the dysregulation of the SV2A levels and the response to antiepileptic medications. SV2A floxed-mice were developed using a cre-lox technique, leading to a strong decrease of SV2A expression in the CA3 field of the hippocampus. We aim here to validate this model using [18F]UCB-H, a novel PET imaging radiotracer with a nanomolar affinity for human SV2A. Methods: In vitro autoradiography were performed on SV2Alox/Cre+ transgenic mouse brain slices. SV2Alox/Cre- mouse was used as control. To obtain a structural reference, brain slices underwent eosin-haematoxylin staining. Images of both procedures were coregistered using π-PMOD software. Regions of interest (Dentate Gyrus, CA1, CA2 and CA3) were drawn according to a stereotaxic atlas of the mouse brain. Results: Analyses showed significant differences in radiotracer binding (p<0.001) between SV2Alox/Cre+ mouse and SV2Alox/Cre- mouse highlighting an important reduction for the labelling density in Ammon's horn, particularly in CA1, compared to Dentate Gyrus where the diminution was less marked. Conclusions: Here, we used the radiotracer [18F]UCB-H to probe the decreased expression of SV2A protein in the hippocampus of SV2Alox/Cre+ mouse versus SV2Alox/Cre- control mouse. Our results contribute to the validation of the model, and encourage us to proceed with further longitudinal and behavioural studies.
Research center :
GIGA CRC (Cyclotron Research Center) In vivo Imaging-Aging \u0026 Memory - ULiège
Disciplines :
Radiology, nuclear medicine & imaging
Life sciences: Multidisciplinary, general & others
Author, co-author :
Serrano Navacerrada, Maria Elisa ;  Université de Liège > Centre de recherches du cyclotron
Becker, Guillaume ;  Université de Liège > Centre de recherches du cyclotron
MENTEN, Catherine ;  Centre Hospitalier Universitaire de Liège - CHU > Génétique
Bahri, Mohamed Ali  ;  Université de Liège > Centre de recherches du cyclotron
Warnier, Corentin ;  Université de Liège > Centre de recherches du cyclotron
Mievis, Frédéric ;  Université de Liège > Centre de recherches du cyclotron
Giacomelli, Fabrice ;  Université de Liège > Centre de recherches du cyclotron
Lemaire, Christian ;  Université de Liège > Centre de recherches du cyclotron
Salmon, Eric  ;  Université de Liège > Département des sciences cliniques > Neuroimagerie des troubles de la mémoire et révalid. cogn.
Luxen, André ;  Université de Liège > Département de chimie (sciences) > Laboratoire de chimie organique de synthèse
Rogister, Bernard  ;  Université de Liège > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, et biochimie humaine
Plenevaux, Alain ;  Université de Liège > Centre de recherches du cyclotron
Language :
English
Title :
EVALUATION OF SV2Alox/Cre TRANSGENIC MOUSE USING [18F]UCB-H IN IN VITRO AUTORADIOGRAPHY
Publication date :
04 September 2015
Number of pages :
Poster
Event name :
International Symposium "Aging and sleep: Perks for longevity?
Event organizer :
G Vandewalle, S Chellappa, P Maquet & Ph Peigneux
Event place :
Liège, Belgium
Event date :
04 septembre 2015
Audience :
International
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique

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