Reference : Phenotypic and genotypic characterisation of Brucella strains isolated from cattle in...
Scientific journals : Article
Life sciences : Veterinary medicine & animal health
Phenotypic and genotypic characterisation of Brucella strains isolated from cattle in the Gambia.
Bankole, A. A. [> >]
Saegerman, Claude mailto [Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Epidémiologie et analyse des risques appl. aux sc. vétér. >]
Berkvens, D. [> >]
Fretin, D. [> >]
Geerts, Sabine mailto [Université de Liège - ULiège > Département de science dentaire > Dentisterie conservatrice de l'adulte - Occlusodontie >]
Ieven, G. [> >]
Walravens, K. [> >]
Veterinary Record : Journal of the British Veterinary Association
Yes (verified by ORBi)
[en] Animals ; Bacterial Typing Techniques ; Brucella/classification/genetics ; Brucella abortus/isolation & purification ; Brucellosis, Bovine/microbiology ; Cattle ; Enzyme-Linked Immunosorbent Assay/veterinary ; Female ; Gambia ; Genotype ; Phenotype ; Rose Bengal/diagnostic use
[en] Thirty-five serum samples and six hygroma fluid samples were collected from sexually mature cattle in one herd with clinical signs of brucellosis (abortion and hygromas) in the Western Region of the Gambia in order to isolate and characterise Brucella species. Information on the sex, age, number of calvings, number of abortions, presence of hygromas, and presence of orchitis was also collected for each animal sampled. Twenty-six (74 per cent) of the serum samples were positive in the rose bengal test and 29 (83 per cent) were positive by indirect ELISA. Three isolates of Brucella, biotyped as Brucella abortus biovar 3, were cultured from six hygroma fluid samples. The multiple locus variable number tandem repeat analysis assay clustered the isolates as B abortus with the same profile for the three isolates, suggesting a common origin of contamination.

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