Article (Scientific journals)
Phenotypic and genotypic characterisation of Brucella strains isolated from cattle in the Gambia.
Bankole, A. A.; Saegerman, Claude; Berkvens, D. et al.
2010In Veterinary Record, 166 (24), p. 753-6
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Keywords :
Animals; Bacterial Typing Techniques; Brucella/classification/genetics; Brucella abortus/isolation & purification; Brucellosis, Bovine/microbiology; Cattle; Enzyme-Linked Immunosorbent Assay/veterinary; Female; Gambia; Genotype; Phenotype; Rose Bengal/diagnostic use
Abstract :
[en] Thirty-five serum samples and six hygroma fluid samples were collected from sexually mature cattle in one herd with clinical signs of brucellosis (abortion and hygromas) in the Western Region of the Gambia in order to isolate and characterise Brucella species. Information on the sex, age, number of calvings, number of abortions, presence of hygromas, and presence of orchitis was also collected for each animal sampled. Twenty-six (74 per cent) of the serum samples were positive in the rose bengal test and 29 (83 per cent) were positive by indirect ELISA. Three isolates of Brucella, biotyped as Brucella abortus biovar 3, were cultured from six hygroma fluid samples. The multiple locus variable number tandem repeat analysis assay clustered the isolates as B abortus with the same profile for the three isolates, suggesting a common origin of contamination.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Bankole, A. A.
Saegerman, Claude  ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Epidémiologie et analyse des risques appl. aux sc. vétér.
Berkvens, D.
Fretin, D.
Geerts, Sabine ;  Université de Liège - ULiège > Département de science dentaire > Dentisterie conservatrice de l'adulte - Occlusodontie
Ieven, G.
Walravens, K.
Language :
English
Title :
Phenotypic and genotypic characterisation of Brucella strains isolated from cattle in the Gambia.
Publication date :
2010
Journal title :
Veterinary Record
ISSN :
0042-4900
eISSN :
2042-7670
Publisher :
British Veterinary Association, United Kingdom
Volume :
166
Issue :
24
Pages :
753-6
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 19 October 2012

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