Bayesian estimation of the true prevalence, sensitivity and specificity of the Rose Bengal and indirect ELISA tests in the diagnosis of bovine brucellosis.
[en] Serology is the most convenient method for detecting brucellosis but the efficient use of such tests in disease control requires evaluation of diagnostic performance and discriminative ability. The objective of this study was to assess the performance of the Rose Bengal test (RBT) and an indirect ELISA (iELISA) in diagnosing brucellosis in 995 serum samples collected from cattle in the Ivory Coast between 2005 and 2009. A Bayesian approach was used to evaluate the two tests by estimating their sensitivities and specificities. The correlation-adjusted sensitivity of the iELISA was estimated to be 96.1% (credibility interval [CrI], 92.7-99.8), whereas that of the RBT was 54.9% (CrI, 23.5-95.1). High correlation-adjusted specificities were found for both tests (95.0%; [CrI, 91.1-99.6] for the iELISA and 97.7%; [CrI, 95.3-99.4] for the RBT, respectively). The true prevalence of brucellosis was estimated from the serum samples to be 4.6% (95%; [CrI, 0.6-9.5]). The level of agreement between the two tests was evaluated using indices of agreement (n=995). Good agreement was found for negative results (96.6%; confidence interval [CI], 95.7-97.4), a finding supported by an estimated significant correlation of 0.37 (95%; CI, 0.01-0.73) within the sera testing negative. Agreement was lower for sera testing positive (52.2% CI: 41.9-62.5). The findings highlight the importance of using these two tests in combination as part of any brucellosis control programme.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Sanogo, Moussa
Thys, Eric
Achi, Yaba L.
Fretin, David
Michel, Patrick
Abatih, Emmanuel
Berkvens, Dirk
Saegerman, Claude ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Epidémiologie et analyse des risques appl. aux sc. vétér.
Language :
English
Title :
Bayesian estimation of the true prevalence, sensitivity and specificity of the Rose Bengal and indirect ELISA tests in the diagnosis of bovine brucellosis.
Publication date :
2013
Journal title :
Veterinary Journal
ISSN :
1090-0233
eISSN :
1532-2971
Publisher :
Elsevier, London, United Kingdom
Volume :
195
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
Copyright (c) 2012 Elsevier Ltd. All rights reserved.
Akakpo A.J., Bornarel P. Epidémiologie des brucelloses animales en Afrique tropicale: Enquêtes clinique, sérologique et bactériologique. Revue Scientifique et Technique de l'Office International des Epizooties 1987, 6:981-1027.
Alton G.G., Jones L.M., Angus R.D., Verger J.M. Techniques for the Brucellosis Laboratory 1988, INRA, Paris, France, pp. 13-85.
Angba A., Traore A., Fritz P. Situation de la brucellose animale en Côte d'Ivoire. Revue d'Elevage et de Médecine Vétérinaire des Pays Tropicaux 1987, 40:325-329.
Bankole A.A., Saegerman C., Berkvens D., Fretin D., Geerts S., Ieven G., Walravens K. Phenotypic and genotypic characterisation of Brucella strains isolated from cattle in the Gambia. Veterinary Record 2010, 166:753-756.
Berkvens D., Speybroeck N., Praet N., Adel A., Lesaffre E. Estimating disease prevalence in a Bayesian framework using probabilistic constraints. Epidemiology 2006, 17:145-153.
Branscum A.J., Gardner I.A., Johnson W.O. Estimation of diagnostic-test sensitivity and specificity through Bayesian modeling. Preventive Veterinary Medicine 2005, 68:145-163.
Brochier B., De Blander H., Hanosset R., Berkvens D., Losson B., Saegerman C. Echinococcus multilocularis and Toxocara canis in urban red foxes (Vulpes vulpes) in Brussels, Belgium. Preventive Veterinary Medicine 2007, 80:65-73.
Camus E. Incidence clinique de la brucellose bovine dans le Nord de la Côte d'Ivoire. Revue d'Elevage et de Médecine Vétérinaire des Pays Tropicaux 1980, 33:263-269.
Cicchetti D., Feinstein A. High agreement but low kappa: II. Resolving the paradoxes. Journal of Clinical Epidemiology 1990, 43:551-558.
Corbel M.J. Brucellosis: An overview. Emerging Infectious Diseases 1997, 3:213-221.
Corbel M.J. Brucellosis in Humans and Animals 2006, WHO Press, World Health Organisation, Genève, Switzerland, pp. 10-35.
Dohoo I.R., Wright P.F., Ruckerbauer G.M., Samagh B.S., Robertson F.J., Forbes L.B. A comparison of five serological tests for bovine brucellosis. Canadian Journal of Veterinary Research 1986, 50:485-493.
Dorny P., Phiri I.K., Vercruysse J., Gabriel S., Willingham A.L., Brandt J., Victor B., Speybroeck N., Berkvens D. A Bayesian approach for estimating values for prevalence and diagnostic test characteristics of porcine cysticercosis. International Journal for Parasitology 2004, 34:569-576.
Enoe C., Georgiadis M.P., Johnson W.O. Estimation of sensitivity and specificity of diagnostic tests and disease prevalence when the true disease state is unknown. Preventive Veterinary Medicine 2000, 45:61-81.
Fosgate G.T., Adesiyun A.A., Hird D.W., Johnson W.O., Hietala S.K., Schurig G.G., Ryan J. Comparison of serologic tests for detection of Brucella infections in cattle and water buffalo (Bubalus bubalis). American Journal of Veterinary Research 2002, 63:1598-1605.
Fredslund J. PHY.FI: Fast and easy online creation and manipulation of phylogeny color figures. BMC Bioinformatics 2006, 7:315.
Gelman A., Rubin D.B. Inference from iterative simulation. Statistical Science 1992, 7:457-511.
Geurden T., Berkvens D., Geldhof P., Vercruysse J., Claerebout E. A Bayesian approach for the evaluation of six diagnostic assays and the estimation of Cryptosporidium prevalence in dairy calves. Veterinary Research 2006, 37:671-682.
Gidel R., Albert J.P., Mao G.L., Retif M. La brucellose en Afrique occidentale et son incidence sur la santé publique. Résultats de dix enquêtes épidémiologiques effectuées en Côte-d'Ivoire, Haute-Volta et Niger de 1970 à 1973. Revue d'Elevage et de Médecine Vétérinaire des Pays Tropicaux 1974, 27:403-418.
Graham P., Bull B. Approximate standard errors and confidence intervals for indices of positive and negative agreement. Journal of Clinical Epidemiology 1998, 51:763-771.
Le Flêche P., Jacques I., Grayon M., Al Dahouk S., Bouchon N.P., Denoeud F., Nockler K., Neubauer H., Guilloteau L.A., Vergnaud G. Evaluation and selection of tandem repeat loci for a Brucella MLVA typing assay. BMC Microbiology 2006, 6:9.
Limet J.N., Kerkhofs P., Wijfels R., Dekeyser P. Le diagnostic sérologique de la brucellose bovine par ELISA. Annales de Médecine Vétérinaire 1988, 132:565-575.
Mangen, M.J., Otte, J., Pfeiffer, D., Chilonda, P., 2002. Bovine Brucellosis in Sub-Saharan Africa: Estimation of Sero-Prevalence and Impact on Meat and Milk Off-take Potential. FAO, Livestock Information and Policy Branch, Livestock Policy Discussion Paper No. 8, Rome, Italy, pp. 39-41.
Maquart M., Le Flêche P., Foster G., Tryland M., Ramisse F., Djønne B., Dahouk S.A., Jacques I., Neubauer H., Walravens K., Godfroid J., Cloeckaert A., Vergnaud G. MLVA-16 typing of 295 marine mammal Brucella isolates from different animal and geographic origins identifies 7 major groups within Brucella ceti and Brucella pinnipedialis. BMC Microbiology 2009, 9:145.
McDermott J.J., Arimi S.M. Brucellosis in sub-Saharan Africa: Epidemiology, control and impact. Veterinary Microbiology 2002, 90:111-134.
Musa M.T., Jahans K.L. The isolation of Brucella melitensis biovar 3 from a testicular hygroma of a ram in a nomadic flock of sheep and goats in western Sudan. Journal of Comparative Pathology 1990, 103:467-470.
MVLABANK, 2010. MLVAbank for Bacterial Genotyping. (accessed 11 January 2011). http://minisatellites.u-psud.fr/MLVAnet.
Nielsen K. Diagnosis of brucellosis by serology. Veterinary Microbiology 2002, 90:447-459.
Ocholi R.A., Kwaga J.K., Ajogi I., Bale J.O. Phenotypic characterization of Brucella strains isolated from livestock in Nigeria. Veterinary Microbiology 2004, 103:47-53.
OIE, 2009. Bovine brucellosis. In: Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Paris, France, pp. 1-35. (accessed 18 May 2011) (Chapter 2.4.3). http://www.oie.int/eng/normes/MMANUAL/2008/pdf/2.04.03_BOVINE_BRUCELL.pdf.
Pilo-Moron E., Pierre F., Kouame J.B. Brucellose bovine en Côte d'Ivoire. Epidémiologie. Revue d'Elevage et de Médecine Vétérinaire des Pays Tropicaux 1979, 32:325-333.
Praet N., Dorny P., Saegerman C., Marcotty T., Berkvens D. Estimation de la prévalence d'une maladie et des caractéristiques des tests diagnostiques par une approche bayésienne. Epidémiologie et Santé Animale 2006, 49:113-130.
Saegerman C., Vo T.K.O., DeWaele L., Gilson D., Bastin A., Dubray G., Flanagan P., Limet J.N., Letesson J.J., Godfroid J. Bovine brucellosis diagnosis by skin test: Conditions for its use and evaluation of its performance. Veterinary Record 1999, 145:214-218.
Saegerman C., De Waele L., Gilson D., Godfroid J., Thiange P., Michel P., Limbourg B., Vo T.K.O., Limet J., Letesson J.J., Berkvens D. Evaluation of three serum i-ELISAs using monoclonal antibodies and protein G as peroxidase conjugate for the diagnosis of bovine brucellosis. Veterinary Microbiology 2004, 100:91-105.
Saegerman, C., Berkvens, D., Godfroid, J., Walravens, K., 2010. Bovine brucellosis. In: Infectious and Parasitic Disease of Livestock, Commonwealth Agricultural Bureau - International, France, pp. 971-1001 (Chapter 77).
Sanogo M., Cissé B., Ouattara M., Walravens K., Praet N., Berkvens D., Thys E. Prévalence réelle de la brucellose bovine dans le centre de la Côte d'Ivoire. Revue d'Elevage et de Médecine Vétérinaire des Pays Tropicaux 2008, 61:147-151.
Shey-Njila O., Daouda N.E., Zoli P.A., Walravens K., Godfroid J., Geerts S. Serological survey of bovine brucellosis in Cameroon. Revue d'Elevage et de Médecine Vétérinaire des Pays Tropicaux 2005, 58:139-143.
Spiegelhalter, D.J., Thomas, A., Best, N., Lunn, D., 2003. WinBUGS Version 1.4 User Manual. http://www.mrc-bsu.cam.ac.uk/bugs/winbugs/manual14.pdf.
Thienpont D., Vandervelden M., Fagard P., Mortelmans J. L'hygroma brucellique: L'aspect clinique caractéristique de la brucellose bovine au Rwanda-Burundi. Revue d'Elevage et de Médecine Vétérinaire des Pays Tropicaux 1961, 14:257-266.
Thys E., Yahaya M.A., Walravens K., Baudoux C., Bagayoko I., Berkvens D., Geerts S. Etude de la prévalence de la brucellose bovine en zone forestière de la Côte d'Ivoire. Revue d'Elevage et de Médecine Vétérinaire des Pays Tropicaux 2005, 58:205-209.
Tounkara K., Maiga S., Traore A., Seck B.M., Akakpo A.J. Epidémiologie de la Brucellose Bovine au Mali: Enquête sérologique et isolement des premières souches de Brucella abortus. Revue Scientifique et Technique de L'Office International des Epizooties 1994, 13:777-786.
Verger J.M., Grayon M., Doutre M.P., Sagna F. Brucella abortus d'origine bovine au Sénégal: Identification et typage. Revue d'Elevage et de Médecine Vétérinaire des Pays Tropicaux 1979, 32:25-32.
Verger J.M., Grayon M., Chantal J., Akakpo J.A. Characteristics of Togo strains of Brucella abortus from cattle. Annales de Recherches Vétérinaires 1982, 13:177-184.
