[en] Penicillium canescens 10-10c strain was cultivated on barley straw hydrolysate as a soluble nutrient source and as inducer for xylanase production. Barley straw hydrolysate was obtained by treatment of barley straw with NaOH or hot water. In shake flask cultures, NaOH treatment was found to increase the biomass production, but was not accompanied by an increase in xylanase production. The best xylanase production (54 U/ml) was observed on hydrolyzed extract from barley straw treated with hot water (100 ºC) for 3 hours. Enzyme production was further improved by scaling up the cultivation process to a 3-L stirred tank bioreactor. For batch cultivations in the bioreactor, the maximum xylanase productivity reached 1.31 and 0.46 U/ml/h, respectively, after 96 and 168 hours of cultivation. However, xylanase productivity reached 3.46 U/ml/h in the continuous culture. These results suggest that xylanase can be produced efficiently by Penicillium canescens 10-10c in continuous culture from an inexpensive source such as barley straw hydrolysate.
Disciplines :
Biotechnology
Author, co-author :
Bakri, Y.
Akeed, Y.
Thonart, Philippe ; Université de Liège - ULiège > Département des sciences de la vie > Biochimie et microbiologie industrielles
Language :
English
Title :
COMPARISON BETWEEN CONTINUOUS AND BATCH PROCESSING TO PRODUCE XYLANASE BY Penicillium canescens 10-10c
Publication date :
2012
Journal title :
Brazilian Journal of Chemical Engineering
ISSN :
0104-6632
eISSN :
1678-4383
Publisher :
Brazilian Society of Chemical Engineering, Sao Polo, Brazil
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