Reference : Heat shock stimulation of a tilapia heat shock protein 70 promoter is mediated by a d...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/13016
Heat shock stimulation of a tilapia heat shock protein 70 promoter is mediated by a distal element
English
Molina, Alfredo [> > > >]
Di Martino, Emmanuel [> > > >]
Martial, Joseph mailto [Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
Muller, Marc mailto [Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
2001
Biochemical Journal
Portland Press
356
Pt 2
353-9
Yes (verified by ORBi)
International
0264-6021
1470-8728
London
United Kingdom
[en] Animals ; Base Sequence ; Binding Sites/genetics ; Carps/genetics ; Cells, Cultured ; DNA/genetics/metabolism ; DNA Primers/genetics ; Gene Expression ; Genes, Reporter ; HSP70 Heat-Shock Proteins/*genetics ; Hot Temperature ; Mutagenesis, Site-Directed ; *Promoter Regions, Genetic ; Sequence Homology, Amino Acid ; Tilapia/*genetics ; Transcription Factors/metabolism ; Zebrafish/embryology/genetics
[en] We reported previously that a tilapia (Oreochromis mossambicus) heat shock protein 70 (HSP70) promoter is able to confer heat shock response on a reporter gene after transient expression both in cell culture and in microinjected zebrafish embryos. Here we present the first functional analysis of a fish HSP70 promoter, the tiHSP70 promoter. Using transient expression experiments in carp EPC (epithelioma papulosum cyprini) cells and in microinjected zebrafish embryos, we show that a distal heat shock response element (HSE1) at approx. -800 is predominantly responsible for the heat shock response of the tiHSP70 promoter. This element specifically binds an inducible transcription factor, most probably heat shock factor, and a constitutive factor. The constitutive complex is not observed with the non-functional, proximal HSE3 sequence, suggesting that both factors are required for the heat shock response mediated by HSE1.
Applied and Fundamental FISH Research Center - AFFISH-RC
http://hdl.handle.net/2268/13016
10.1042/0264-6021:3560353
http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=11368761
2001/05/23
http://www.portlandpress.com/pp/default.htm

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