Article (Scientific journals)
Heat shock stimulation of a tilapia heat shock protein 70 promoter is mediated by a distal element
Molina, Alfredo; Di Martino, Emmanuel; Martial, Joseph et al.
2001In Biochemical Journal, 356 (Pt 2), p. 353-9
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Keywords :
Animals; Base Sequence; Binding Sites/genetics; Carps/genetics; Cells, Cultured; DNA/genetics/metabolism; DNA Primers/genetics; Gene Expression; Genes, Reporter; HSP70 Heat-Shock Proteins/*genetics; Hot Temperature; Mutagenesis, Site-Directed; *Promoter Regions, Genetic; Sequence Homology, Amino Acid; Tilapia/*genetics; Transcription Factors/metabolism; Zebrafish/embryology/genetics
Abstract :
[en] We reported previously that a tilapia (Oreochromis mossambicus) heat shock protein 70 (HSP70) promoter is able to confer heat shock response on a reporter gene after transient expression both in cell culture and in microinjected zebrafish embryos. Here we present the first functional analysis of a fish HSP70 promoter, the tiHSP70 promoter. Using transient expression experiments in carp EPC (epithelioma papulosum cyprini) cells and in microinjected zebrafish embryos, we show that a distal heat shock response element (HSE1) at approx. -800 is predominantly responsible for the heat shock response of the tiHSP70 promoter. This element specifically binds an inducible transcription factor, most probably heat shock factor, and a constitutive factor. The constitutive complex is not observed with the non-functional, proximal HSE3 sequence, suggesting that both factors are required for the heat shock response mediated by HSE1.
Research center :
AFFISH-RC - Applied and Fundamental FISH Research Center - ULiège
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Molina, Alfredo
Di Martino, Emmanuel
Martial, Joseph ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Muller, Marc  ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Language :
English
Title :
Heat shock stimulation of a tilapia heat shock protein 70 promoter is mediated by a distal element
Publication date :
2001
Journal title :
Biochemical Journal
ISSN :
0264-6021
eISSN :
1470-8728
Publisher :
Portland Press, London, United Kingdom
Volume :
356
Issue :
Pt 2
Pages :
353-9
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
http://www.portlandpress.com/pp/default.htm
Available on ORBi :
since 20 May 2009

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