Heat shock stimulation of a tilapia heat shock protein 70 promoter is mediated by a distal element

Animals; Base Sequence; Binding Sites/genetics; Carps/genetics; Cells, Cultured; DNA/genetics/metabolism; DNA Primers/genetics; Gene Expression; Genes, Reporter; HSP70 Heat-Shock Proteins/*genetics; Hot Temperature; Mutagenesis, Site-Directed; *Promoter Regions, Genetic; Sequence Homology, Amino Acid; Tilapia/*genetics; Transcription Factors/metabolism; Zebrafish/embryology/genetics

Abstract :

[en] We reported previously that a tilapia (Oreochromis mossambicus) heat shock protein 70 (HSP70) promoter is able to confer heat shock response on a reporter gene after transient expression both in cell culture and in microinjected zebrafish embryos. Here we present the first functional analysis of a fish HSP70 promoter, the tiHSP70 promoter. Using transient expression experiments in carp EPC (epithelioma papulosum cyprini) cells and in microinjected zebrafish embryos, we show that a distal heat shock response element (HSE1) at approx. -800 is predominantly responsible for the heat shock response of the tiHSP70 promoter. This element specifically binds an inducible transcription factor, most probably heat shock factor, and a constitutive factor. The constitutive complex is not observed with the non-functional, proximal HSE3 sequence, suggesting that both factors are required for the heat shock response mediated by HSE1.

Research Center/Unit :

AFFISH-RC - Applied and Fundamental FISH Research Center - ULiège

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Molina, Alfredo

Di Martino, Emmanuel

Martial, Joseph ; Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire

Muller, Marc ; Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire

Language :

English

Title :

Heat shock stimulation of a tilapia heat shock protein 70 promoter is mediated by a distal element

Publication date :

2001

Journal title :

Biochemical Journal

ISSN :

0264-6021

eISSN :

1470-8728

Publisher :

Portland Press, London, United Kingdom

Volume :

356

Issue :

Pt 2

Pages :

353-9

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=11368761

Commentary :

http://www.portlandpress.com/pp/default.htm

Available on ORBi :

since 20 May 2009

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