[en] Organochlorine pesticides such as endosulfan have been shown to have both lethal and sublethal effects on amphibians. In this context, behavioral endpoints have proved their usefulness in evidencing impacts of such chemicals at environmental concentrations that do not necessarily cause mortality. The recent development of video-tracking technologies now offers the possibility of accurately quantifying locomotor behaviors. However, these techniques have not yet been applied to evaluating the toxicity of pesticides in amphibians. We therefore aimed at determining the potential toxicity of endosulfan on endpoints associated with locomotion after short-term environmental endosulfan exposure in Rana temporaria tadpoles and at using these data as warning systems for survival alterations after a longer exposure. To this end, we analyzed video-tracks of 64 tadpoles (two pesticide treatments: 5 and 50 μg L−1, one control and one solvent-control) with Ethovision XT 7 software. The highest endosulfan concentration had a significant effect on all four behavioral endpoints. Contaminated tadpoles traveled shorter distances, swam less often, at a lower mean speed, and occupied a less peripherical position than control tadpoles. The lowest endosulfan concentration had similar but lower effects, and did not affect mean speed during swimming. Survival was reduced only after a long-term exposure to endosulfan and was associated with short-term behavioral dysfunctions. These results show that endosulfan strongly affects the behavioral repertory of amphibian tadpoles, but in different ways depending on concentration, thus suggesting that the pesticide has complex modes of action. Given the importance of locomotion and space use in tadpole success in their aquatic environment, these results confirm the toxic action of endosulfan. By highlighting effects before mortality markers, video-tracking systems also show their potential as sentinels of sublethal effects of pesticides.
Centre/Unité de recherche :
AFFISH-RC - Applied and Fundamental FISH Research Center - ULiège
Denoël, Mathieu ; Université de Liège - ULiège > Département de Biologie, Ecologie et Evolution > Biologie du comportement - Ethologie et psychologie animale
Libon, Sylvie; Université de Liège - ULiège > Département de Biologie, Ecologie Evolution > Unité de Biologie du Comportement
Kestemont, Patrick; Facultés Universitaires Notre-Dame de la Paix - Namur - FUNDP > Research Unit in Environmental and Evolutionary Biology
Brasseur, Catherine ; Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.)
Focant, Jean-François ; Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique, organique et biologique
De Pauw, Edwin ; Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.)
Langue du document :
Anglais
Titre :
Effects of a sublethal pesticide exposure on locomotor behavior: A video-tracking analysis in larval amphibians
Date de publication/diffusion :
2013
Titre du périodique :
Chemosphere
ISSN :
0045-6535
eISSN :
1879-1298
Maison d'édition :
Elsevier Science, Oxford, Royaume-Uni
Volume/Tome :
90
Fascicule/Saison :
3
Pagination :
945-951
Peer reviewed :
Peer reviewed vérifié par ORBi
Organisme subsidiant :
FRFC - Fonds de la Recherche Fondamentale Collective F.R.S.-FNRS - Fonds de la Recherche Scientifique
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