Reference : Effects of a sublethal pesticide exposure on locomotor behavior: A video-tracking ana...
Scientific journals : Article
Social & behavioral sciences, psychology : Animal psychology, ethology & psychobiology
Life sciences : Environmental sciences & ecology
Life sciences : Aquatic sciences & oceanology
Life sciences : Agriculture & agronomy
Effects of a sublethal pesticide exposure on locomotor behavior: A video-tracking analysis in larval amphibians
Denoël, Mathieu mailto [Université de Liège - ULiège > Département de Biologie, Ecologie et Evolution > Biologie du comportement - Ethologie et psychologie animale >]
Libon, Sylvie [Université de Liège - ULiège > Département de Biologie, Ecologie Evolution > Unité de Biologie du Comportement > >]
Kestemont, Patrick [Facultés Universitaires Notre-Dame de la Paix - Namur - FUNDP > > Research Unit in Environmental and Evolutionary Biology > >]
Brasseur, Catherine mailto [Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.) >]
Focant, Jean-François mailto [Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique, organique et biologique >]
De Pauw, Edwin mailto [Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.) >]
Elsevier Science
Yes (verified by ORBi)
United Kingdom
[en] Amphibian decline ; Behavioural endpoints ; Behavioral endpoints ; Biomarker ; Endosulfan ; POP ; Survival ; Video-tracking ; Activity ; Speed ; Distance moved ; Space use ; Behavioral conservation ; Mechanisms ; Organochlorine pesticide ; Ethanol ; Solvent ; Locomotion ; Noldus Ethovision XT ; Short-term ; Persistent organic pollutant
[fr] Comportement ; Marqueur comportemental ; Amphibien ; Tetard ; Mouvement ; Survie
[en] Organochlorine pesticides such as endosulfan have been shown to have both lethal and sublethal effects on amphibians. In this context, behavioral endpoints have proved their usefulness in evidencing impacts of such chemicals at environmental concentrations that do not necessarily cause mortality. The recent development of video-tracking technologies now offers the possibility of accurately quantifying locomotor behaviors. However, these techniques have not yet been applied to evaluating the toxicity of pesticides in amphibians. We therefore aimed at determining the potential toxicity of endosulfan on endpoints associated with locomotion after short-term environmental endosulfan exposure in Rana temporaria tadpoles and at using these data as warning systems for survival alterations after a longer exposure. To this end, we analyzed video-tracks of 64 tadpoles (two pesticide treatments: 5 and 50 μg L−1, one control and one solvent-control) with Ethovision XT 7 software. The highest endosulfan concentration had a significant effect on all four behavioral endpoints. Contaminated tadpoles traveled shorter distances, swam less often, at a lower mean speed, and occupied a less peripherical position than control tadpoles. The lowest endosulfan concentration had similar but lower effects, and did not affect mean speed during swimming. Survival was reduced only after a long-term exposure to endosulfan and was associated with short-term behavioral dysfunctions. These results show that endosulfan strongly affects the behavioral repertory of amphibian tadpoles, but in different ways depending on concentration, thus suggesting that the pesticide has complex modes of action. Given the importance of locomotion and space use in tadpole success in their aquatic environment, these results confirm the toxic action of endosulfan. By highlighting effects before mortality markers, video-tracking systems also show their potential as sentinels of sublethal effects of pesticides.
Applied and Fundamental FISH Research Center - AFFISH-RC
Fonds de la Recherche Fondamentale Collective - FRFC ; Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS
Researchers ; Professionals ; Students
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