Reference : Evaluation of chlorine containing cellulose-based chiral stationary phases for the LC...
Scientific journals : Article
Human health sciences : Pharmacy, pharmacology & toxicology
Evaluation of chlorine containing cellulose-based chiral stationary phases for the LC enantioseparation of basic pharmaceuticals using polar non-aqueous mobile phases.
Dossou, Katina Sourou Sylvestre [Université de Liège - ULiège > > > Doct. sc. bioméd. & pharma. (Bologne)]
CHIAP, Patrice mailto [Centre Hospitalier Universitaire de Liège - CHU > > Pharmacologie clinique >]
Servais, Anne-Catherine mailto [Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments >]
Fillet, Marianne mailto [Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments >]
Crommen, Jacques mailto [Université de Liège - ULiège > Département de pharmacie > Département de pharmacie >]
Journal of Separation Science
Yes (verified by ORBi)
[en] Acidic additive ; Discrimination ability ; Enantiomers ; Polysaccharide-based chiral stationary phases
[en] The discrimination ability of three cellulose-based chiral stationary phases (CSPs) was evaluated towards the enantiomers of basic drugs, using ACN as the main solvent in polar organic mobile phases. The study was focused on CSPs containing cellulose tris(3-chloro-4-methylphenylcarbamate) (3-Cl-4-MePC), cellulose tris(4-chloro-3-methylphenylcarbamate) (4-Cl-3-MePC) or cellulose tris(3,5-dichlorophenylcarbamate) (3,5-diClPC) as the chiral selector. The behaviour of these CSPs was studied systematically in order to investigate the influence of the presence and position of the chlorine substituents on the phenylcarbamate moieties on the retention and resolution of the enantiomers. The evaluation was made with three different generic mobile phases, namely ACN/0.1%DEA/0.1% TFA (DEA, diethylamine), ACN/0.1%DEA/0.2% FA and ACN/0.1%DEA/0.2%AcA, deduced from the previous study. The nature of the acidic additive and of the chiral selector was found to be particularly important for the retention and enantioresolution of these basic compounds. High-resolution values could be obtained for most studied enantiomers with these CSPs, clearly demonstrating the interest of using them in combination with polar organic mobile phases. However, significant differences in enantioresolution between the CSPs have been observed for many compounds, indicating that these phases seem to be quite complementary.
Alice Seghers Foundation of University of Liege ; Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS
Copyright (c) 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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