[en] Mass spectrometry (MS)-based technology provides label-free localization of molecules in tissue samples. Drugs, proteins, lipids and metabolites can easily be monitored in their environment. Resolution can be achieved down to the cellular level (10–20 mm) for conventional matrix-assisted laser desorption/ionization (MALDI) imaging, or even to the subcellular level for more complex technologies such as secondary ionization mass spectrometry (SIMS) imaging. One question remains: are we going to be able to investigate functional relationships between drugs and proteins and compare with localized phenomena? This review describes the various spatial levels of investigation offered by mass spectrometry imaging (MSI), and the advantages and disadvantages compared with other labeling technologies.
Research Center/Unit :
Giga-Systems Biology and Chemical Biology - ULiège CRO2 (Marseille)
Disciplines :
Human health sciences: Multidisciplinary, general & others Life sciences: Multidisciplinary, general & others Chemistry
Author, co-author :
Ait-Belkacem, Rima; Aix-Marseille Université > CRO2, 13385 Marseille, France > INSERM, UMR 911, 13385 Marseille, France
Sellami, Lyna; Aix-Marseille Université > CRO2, 13385 Marseille, France > INSERM, UMR 911, 13385 Marseille, France
Villard, Claude; Aix-Marseille Université > CR02, 13385 Marseille, France > INSERM, UMR 911, 13385 Marseille, France
De Pauw, Edwin ; Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.)
Calligaris, David ; Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.)
Lafitte, Daniel; Aix-Marseille Université > CRO2, 13385, Marseille, France > INSERM, UMR 911, 13385 Marseille, France
Language :
English
Title :
Mass spectrometry imaging is moving toward drug protein co-localization
INSERM - Institut National de la Santé et de la Recherche Médicale F.R.S.-FNRS - Fonds de la Recherche Scientifique ULiège - Université de Liège FEDER - Fonds Européen de Développement Régional ARC - Association pour la Recherche sur le Cancer Canceropôle PACA Shimadzu
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