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Evolutionary analysis of a complex multigene family by semi-automated mining of public sequence databases
Baurain, Denis
2012Systems Biology Workshop of the Greater Region University
 

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Abstract :
[en] Eukaryotic genes have a mosaic structure of exons and introns. After transcription, precursor mRNAs have to be spliced to yield mature mRNAs suitable for protein synthesis. This process is termed splicing and is carried out by a complex known as the spliceosome. Serine/arginine-rich (SR) proteins play essential roles in splicing. They have a modular organization featuring at least one RNA recognition motif (RRM) domain and a carboxyl-terminal RS region enriched in arginine/serine dipeptides. However, their architectures are quite diverse, which has so far complicated their evolutionary analysis. To investigate the origin and evolution of SR splicing factors, we inferred phylogenies for more than 12,000 RRM domains representing more than 200 broadly sampled organisms. Our results show that all SR proteins share a single ancient origin. Based on refined analyses, we propose a scenario for their diversification into four natural families and a dozen subfamilies. Altogether, this work confirms the homogeneity and antiquity of SR splicing factors while establishing robust phylogenetic relationships between animal and plant proteins. In this talk, I will focus on the bioinformatics approaches required to carry out such a large-scale phylogenetic analysis.
Disciplines :
Biochemistry, biophysics & molecular biology
Genetics & genetic processes
Author, co-author :
Baurain, Denis  ;  Université de Liège - ULiège > Département des sciences de la vie > Phylogénomique des eucaryotes
Language :
English
Title :
Evolutionary analysis of a complex multigene family by semi-automated mining of public sequence databases
Publication date :
19 June 2012
Event name :
Systems Biology Workshop of the Greater Region University
Event place :
Lultzhausen, Luxembourg
Event date :
19-20 June 2012
By request :
Yes
Audience :
International
Available on ORBi :
since 22 June 2012

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