Occurrence of cardiovascular calcifications in normal, aging rats.

[en] BACKGROUND: Cardiovascular calcification is an independent predictor of morbidity and mortality and increases with age. Animal models are frequently used to investigate the underlying pathophysiology. Only scarce data regarding the effect of aging on calcifications in these animal models are available. The aim of this study is to investigate the occurrence of cardiovascular calcifications in normal, aging rats. METHODS: A mixed inbred/outbred population of 44 male Lewis/Wistar rats was studied. Group 1 of three-month-old rats, group 2 twelve-month-old, group 3 twenty-four-month-old and group 4 thirty-month-old rats. Calibrated integrated backscatter (cIB) values and blood parameters (creatinine, parathyroid hormone (PTH)) were measured, followed by ex-vivo micro-CT and histology as reference methods. RESULTS: Cardiovascular calcifications developed with age, as demonstrated by significantly increasing cIB values of the aortic valve and myocardium. This was confirmed by a significant increase in the calcified volume on ex-vivo micro-CT and in the histological calcium score. There was also a significantly higher level of creatinine and PTH with age. CONCLUSIONS: As in humans, cardiovascular calcifications progressively increase with age in the normal rat. Therefore the aging rat model could be used for studying calcifying cardiovascular disease. cIB might have a value in future studies for the early detection of subclinical calcifications in humans.

Disciplines :

Cardiovascular & respiratory systems

Author, co-author :

Roosens, Bram

Bala, Gezim

Droogmans, Steven

Hostens, Jeroen

Somja, Joan ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques

Delvenne, Eléonore

Schiettecatte, J

Delvenne, Philippe ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques

Lahoutte, T

Van Camp, G

Cosyns, Bernard

Language :

English

Title :

Occurrence of cardiovascular calcifications in normal, aging rats.

Publication date :

2012

Journal title :

Experimental Gerontology

ISSN :

0531-5565

eISSN :

1873-6815

Publisher :

Elsevier Science, Tarrytown, United States - New York

Volume :

Issue :

Pages :

614-9

Peer reviewed :

Peer Reviewed verified by ORBi

Commentary :

Available on ORBi :

since 20 June 2012

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